Antitumor Effect of By-1 from Spent Broth from Submerged Cultures of Stout Camphor Medicinal Mushroom, Taiwanofungus camphoratus (Higher Basidiomycetes), on A549 Adenocarcinoma Cells

文献类型: 外文期刊

第一作者: Wang, Wen-Han

作者: Wang, Wen-Han;Wang, Li-Yuan;Zhang, He-Nan;Zhang, Jing-Song;Jia, Wei;Wang, Wen-Han;Wang, Li-Yuan;Zhang, He-Nan;Zhang, Jing-Song;Jia, Wei;Yang, Hai-Rui;Zhang, Ya-Ping;Fan, Hua;Zhao, Xiang-Li

作者机构:

关键词: anti-lung cancer;By-1;lung adenocarcinoma;medicinal mushrooms;Taiwanofungus camphoratus

期刊名称:INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS ( 影响因子:1.921; 五年影响因子:1.879 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: By-1 was obtained from spent broth from submerged cultures of Taiwanofungus camphoratus. This report evaluates the effects of By-1 on plate clone formation, wound healing, cell cycle, activated caspase-3 expression, and ROS release in A549 lung cancer cells. The result of plate clone formation assay revealed that By-1 could dramatically inhibit the viability of A549 cells in vitro. The inhibitory effect of By-1 on cell migration was tested using a wound healing assay. Proliferation rates of A549 cells were significantly inhibited following exposure to By-1 (12.5, 50, and 80 mu g/mL). Flow cytometry revealed that the extracts increased, in a concentration-dependent manner, the number of cells in the G0/G1 phases of the cell cycle. The results of the caspase-3 experiment suggested that By-1 could induce A549 cells apoptosis, and this apoptosis was related to the release of reactive oxygen species by the A549 cells. All these results indicate that By-1 has potential in anti-lung cancer drug development.

分类号: Q94

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