Microbial Secondary Metabolite, Phlegmacin B-1, as a Novel Inhibitor of Insect Chitinolytic Enzymes

文献类型： 外文期刊

第一作者： Liu, Tian

作者： Liu, Tian;Duan, Yanwei;Yang, Qing;Chen, Lei;Liu, Tian;Duan, Yanwei;Yang, Qing;Liu, Tian;Yang, Qing;Lu, Xinhua

作者机构：

关键词： chitinase;hexosaminidase;inhibitor;phlegmacin;microbial secondary metabolites

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： Periodic chitin remodeling during insect growth and development requires a synergistic action of two glycosyl hydrolase (GH) family enzymes, GH18 chitinase and GH2O beta-N-acetylhexosaminidase (Hex). Inhibiting either or both of these enzymes is a promising strategy for pest control and management. In this study, Of Chi-h (a GH18 chitinase) and OfHexl (a GH2O Hex) from Ostrinia furnacalis were used to screen a library of microbial secondary metabolites. Phlegmacin B, was found to be the inhibitor of both Of Chi-h and OfHexl with K-i varues of 5.5 mu M and 26 mu M, respectively. Injection and feeding experiments demonstrated that phlegmacin B-1 has insecticidal effect on 0. furnacalis's larvae. Phlegmacin B-1 was predicted to bind to the active pockets of both Of Chi-h and OfHexl. Phlegmacin B, also showed moderate inhibitory activities against other bacterial and insect GH18 enzymes. This work provides an example of exploiting microbial secondary metabolites as potential pest control and management agents.

分类号： R15`S

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