A Practical Method for Peach-related Species Identification and Hybrid Analysis Using Simple Sequence Repeat Markers

文献类型： 外文期刊

第一作者： Cai, Zuguo

作者： Cai, Zuguo;Zeng, Wenfang;Niu, Liang;Lu, Zhenhua;Cui, Guochao;Zhu, Yunqin;Pan, Lei;Ding, Yifeng;Wang, Zhiqiang;Cai, Zuguo

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期刊名称：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE （ 影响因子：1.144； 五年影响因子：1.617 ）

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收录情况： SCI

摘要： Cultivated peach (Prunus persica) is an important fruit species worldwide. The wild relatives in Prunus, such as P. mira, P. davidiana, P. kansuensis, P. ferganensis, and P. persica, are valuable for peach breeding, and early and accurate identification of parental and hybrid genotypes is critical. In this study, 20 representative accessions of peach germplasm from the National Germplasm Repository of Peach in China were used to select a set of 18 simple sequence repeat (SSR) markers for accurate species discrimination. Eight unknown peach samples were successfully identified using the SSR panel and species genotype database. Interspecific hybrid genotypes of P. persica x P. davidiana, P. persica x P. kansuensis, and P. persica x P. ferganensis were also analyzed reliably. The markers were amenable to high-throughput fluorescent labeling and capillary electrophoresis (CE) analysis, allowing rapid and efficient species identification. The practical method described in this study will facilitate peach breeding and germplasm management.

分类号： S6

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