Alleviation of enterotoxigenic Escherichia coli challenge by recombinant Lactobacillus plantarum expressing a FaeG- and DC-targeting peptide fusion protein

文献类型： 外文期刊

第一作者： Yang, G.

作者： Yang, G.;Jiang, Y.;Li, C.;Yang, W.;Hu, J.;Ye, L.;Shi, C.;Wang, C.;Tong, P.;Gu, W.;Shan, B.

作者机构：

关键词： ETEC;FaeG;DC-targeting peptide;Lactobacilus plantarum;immune response

期刊名称：BENEFICIAL MICROBES （ 影响因子：4.205； 五年影响因子：4.252 ）

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年卷期：

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收录情况： SCI

摘要： FaeG is the major subunit of K88 fimbriae. These cell surface attachments are considered to be the major virulence factor of enterotoxigenic Escherichia coli (ETEC), which causes diarrhoea in piglets. The use of dendritic cell-targeting peptide (DCpep) has been demonstrated to be an effective approach to enhance the immunity of vaccines. Lactobacillus plantarum is an attractive candidate for oral vaccination owing to its beneficial effects and safety. In this study, L. plantarum was employed to deliver a FaeG-DCpep fusion antigen, and the immune response in mice was evaluated. The synthesis of FaeG-DCpep dramatically increased the adhesion of recombinant L. plantarum (RLP) to IPEC-J2 cell surfaces, resulting in direct competition between L. plantarum and ETEC during adhesion assays. Significantly higher levels of body weight gain, sera immunoglobulin G and intestinal immunoglobulin A were observed in BALB/c mice immunised with RLP. In addition, the number of CD19(+) B cells and CD11c(+) DC cells and the expression levels of several cytokines in the spleen and lymph nodes increased significantly compared to non-immunised mice. The oral administration of RLP also alleviated the symptoms of ETEC challenge, as shown by haematoxylin-eosin staining, indicating that RLP may be an efficient vaccine candidate.

分类号： Q93

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