Evaluation of two strains of Mareles disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus

文献类型： 外文期刊

第一作者： Li, Kai

作者： Li, Kai;Liu, Yongzhen;Liu, Changjun;Gao, Li;Gao, Yulong;Zhang, Yanping;Cui, Hongyu;Qi, Xiaole;Zhong, Li;Wang, Xiaomei

作者机构：

关键词： Marek's disease virus;Infectious bursal disease virus;VP2 gene;MDVI vaccine strains;Protective efficacy

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：5.97； 五年影响因子：5.801 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Attenuated strains of Marek's disease virus serotype 1 (MDVI), and the closely related herpesvirus of turkeys, are among the most potent vectors for development of recombinant vaccines for poultry. To investigate the effects of MDVI strain characteristics on the protective efficacy of the recombinant vaccines, we developed two recombinant MDVI vaccines for expressing the VP2 gene of infectious bursal disease virus (IBDV) based on two different MDVI strains, the attenuated strain 814 and the Meq gene deleted recombinant MDVI strain rLMS Delta Meq, as the viral vectors. The r814-VP2 virus based on the 814 strain exhibited higher replication efficiency in cell culture while lower viral titers in chickens, compared to rLMS Delta Meq-VP2 derived from the rLMS Delta Meq strain. Further studies indicated that r814-VP2 produced higher levels of VP2 protein in cells and elicited stronger immune responses against IBDV in chickens than rLMS Delta Meq-VP2. After IBDV challenge, rLMS Delta Meq-VP2 provided 50% protection against mortality, and the birds that survived developed bursa] atrophy and gross lesions. In contrast, r814-VP2 conferred complete protection not only against development of clinical signs and mortality, but also against the formation of bursal lesions. The results indicate that different MDVI vector influences the protective efficacy of recombinant MDVI vaccines. The r814-VP2 has the potential to serve as a bivalent vaccine against two important lethal pathogens of chickens.(C) 2016 Elsevier B.V. All rights reserved.

分类号： R37

  • 相关文献

[1]Deletion of the BAC sequences from recombinant meq-null Marek's disease (MD) virus increases immunosuppression while maintaining protective efficacy against MD. Su, S.,Cui, N.,Sun, P.,Cui, Z.,Li, J.,Li, H.,Li, Y..

[2]Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious bursal disease virus. Li, Kai,Liu, Yongzhen,Liu, Changjun,Gao, Li,Zhang, Yanping,Gao, Yulong,Cui, Hongyu,Qi, Xiaole,Zhong, Li,Wang, Xiaomei.

[3]Establishment and evaluation of a novel TaqMan probe-based real-time PCR for detection of Canine Parvovirus. Shi, Lijun,Zhao, Zhanzhong,Wang, Jing,Yuan, WeiFeng,Zhu, Hongfei,Li, Gang,Yin, Huiqiong,Zhang, Jingang. 2012

[4]Phylogenetic analysis of the VP2 gene of Aleutian mink disease parvoviruses isolated from 2009 to 2011 in China. Sang, Yu,Hou, Zhijun,Zhang, Yanlong,Ma, Jian.

[5]Phylogenetic Analysis of Canine Parvovirus VP2 Gene in China. Yi, L.,Tong, M.,Cheng, Y.,Cheng, S.,Song, W..

[6]Evidence for natural recombination in the capsid gene VP2 of Taiwanese goose parvovirus. Wang, Shao,Cheng, Xiaoxia,Chen, Shaoying,Lin, Fengqiang,Chen, Shilong,Zhu, Xiaoli,Wang, Jingxiang,Wang, Shao,Cheng, Xiaoxia,Chen, Shaoying,Lin, Fengqiang,Chen, Shilong,Zhu, Xiaoli,Wang, Jingxiang.

[7]Phylogenetic Analysis of Canine Parvovirus Isolates from Sichuan and Gansu Provinces of China in 2011. Xu, J.,Guo, H. -C.,Wei, Y. -Q.,Shu, L.,Sun, S. -Q.,Xu, J.,Shu, L.,Li, J. -S.,Cao, S. -Z.,Wang, J..

[8]High resolution melting curve analysis as a new tool for rapid identification of canine parvovirus type 2 strains. Bingga, Gali,Ding, Shuangyang,Bingga, Gali,Liu, Zhicheng,Zhang, Jianfeng,Lin, Lifeng,Zhu, Yujun,Guo, Pengju.

[9]Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis. Pan, Y,Cai, H,Li, SX,Tian, X,Li, T,Zhu, YX. 2003

[10]Expression of truncated Babesia microti apical membrane protein 1 and rhoptry neck protein 2 and evaluation of their protective efficacy. Wang, Guanbo,Efstratiou, Artemis,Moumouni, Paul Franck Adjou,Liu, Mingming,Jirapattharasate, Charoonluk,Guo, Huanping,Gao, Yang,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Zhou, Mo.

[11]Protective efficacy of a novel recombinant Marek's disease virus vector vaccine against infectious bursal disease in chickens with or without maternal antibodies. Li, Kai,Liu, Yongzhen,Zhang, Yanping,Gao, Li,Liu, Changjun,Cui, Hongyu,Qi, Xiaole,Gao, Yulong,Zhong, Li,Wang, Xiaomei.

[12]Protective Efficacy of the Inactivated H5N1 Influenza Vaccine Re-6 Against Different Clades of H5N1 Viruses Isolated in China and the Democratic People's Republic of Korea. Zeng, Xianying,Deng, Guohua,Liu, Liling,Li, Yanbing,Shi, Jianzhong,Chen, Pucheng,Feng, Huapeng,Tian, Guobin,Chen, Hualan,Zeng, Xianying,Deng, Guohua,Liu, Liling,Li, Yanbing,Shi, Jianzhong,Chen, Pucheng,Feng, Huapeng,Tian, Guobin,Chen, Hualan,Liu, Jingli,Guo, Xingfu,Mao, Shenggang,Yang, Fan,Chen, Zhiyu.

[13]Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation. Wen, Feng,Ma, Ji-Hong,Yu, Hai,Yang, Fu-Ru,Huang, Meng,Zhou, Yan-Jun,Li, Ze-Jun,Tong, Guang-Zhi.

[14]Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine. Zheng, Haixue,Lian, Kaiqi,Yang, Fan,Jin, Ye,Zhu, Zixiang,Guo, Jianhong,Cao, Weijun,Liu, Huanan,He, Jijun,Zhang, Keshan,Li, Dan,Liu, Xiangtao.

[15]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[16]Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens. Li, Huixin,Wang, Yulong,Han, Zongxi,Wang, Yu,Liang, Shulin,Jiang, Lu,Kong, Xiangang,Liu, Shengwang,Wang, Yulong,Hu, Yonghao.

[17]Protective efficacy of pVAX-RON5p against acute and chronic infections of Toxoplasma gondii in BALB/c mice. Zhao, Yu,Li, Zhong-Yuan,Chen, Jia,Liu, Shan-Shan,Zhu, Xing-Quan,Zhou, Dong-Hui,Zhao, Yu,Sun, Xiao-Lin,Li, Zhong-Yuan,Liu, Shan-Shan,Zhu, Xing-Quan.

[18]Protective Efficacy of an H5N1 Inactivated Vaccine Against Challenge with Lethal H5N1, H5N2, H5N6, and H5N8 Influenza Viruses in Chickens. Tian, Guobin,Chen, Hualan.

[19]Construction and immune effect of Haemophilus parasuis DNA vaccine encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in mice. Bei, Weicheng,Fu, Shulin,Ou, Jiwen,Tan, Chen,Chen, Huanchun,Bei, Weicheng,Zhang, Minmin,Liu, Jinlin. 2012

[20]Co-Infection with Marek's Disease Virus and Reticuloendotheliosis Virus Increases Illness Severity and Reduces Marek's Disease Vaccine Efficacy. Sun, Guo-Rong,Zhang, Yan-Ping,Zhou, Lin-Yi,Lv, Hong-Chao,Zhang, Feng,Li, Kai,Gao, Yu-Long,Qi, Xiao-Le,Cui, Hong-Yu,Wang, Yong-Qiang,Gao, Li,Pan, Qing,Wang, Xiao-Mei,Liu, Chang-Jun. 2017

作者其他论文 更多>>

微信小程序