Mimic Phosphorylation of a beta C1 Protein Encoded by TYLCCNB Impairs Its Functions as a Viral Suppressor of RNA Silencing and a Symptom Determinant

文献类型： 外文期刊

第一作者： Zhong, Xueting

作者： Zhong, Xueting;Wang, Zhan Qi;Xiao, Ruyuan;Cao, Linge;Wang, Yaqin;Xie, Yan;Zhou, Xueping;Zhou, Xueping

作者机构：

关键词： TYLCCNB-beta C1;Nicotiana benthamiana;SnRK1;geminivirus;host defense factor;posttranscriptional gene silencing;protein phosphorylation;transcriptional gene silencing

期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.103； 五年影响因子：5.078 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Phosphorylation of the beta C1 protein encoded by the betasatellite of tomato yellow leaf curl China virus (TYLCCNB-beta C1) by SNF1-related protein kinase 1 (SnRK1) plays a critical role in defense of host plants against geminivirus infection in Nicotiana benthamiana. However, how phosphorylation of TYLCCNB-beta C1 impacts its pathogenic functions during viral infection remains elusive. In this study, we identified two additional tyrosine residues in TYLCCNB-beta C1 that are phosphorylated by SnRK1. The effects of TYLCCNB-beta C1 phosphorylation on its functions as a viral suppressor of RNA silencing (VSR) and a symptom determinant were investigated via phosphorylation mimic mutants in N. benthamiana plants. Mutations that mimic phosphorylation of TYLCCNB-beta C1 at tyrosine 5 and tyrosine 110 attenuated disease symptoms during viral infection. The phosphorylation mimics weakened the ability of TYLCCNB-beta C1 to reverse transcriptional gene silencing and to suppress posttranscriptional gene silencing and abolished its interaction with N. benthamiana ASYMMETRIC LEAVES 1 in N. benthamiana leaves. The mimic phosphorylation of TYLCCNB-beta C1 had no impact on its protein stability, subcellular localization, or self-association. Our data establish an inhibitory effect of phosphorylation of TYLCCNB-beta C1 on its pathogenic functions as a VSR and a symptom determinant and provide a mechanistic explanation of how SnRK1 functions as a host defense factor.

分类号： Q934.2

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