Effects of ultrasound-assisted alkaline extraction on the physiochemical and functional characteristics of chicken liver protein isolate

文献类型: 外文期刊

第一作者: Zou, Y.

作者: Zou, Y.;Li, P. P.;Zhang, M. H.;Sun, Z. L.;Sun, C.;Geng, Z. M.;Xu, W. M.;Wang, D. Y.;Zhang, K.;Wang, L.

作者机构:

关键词: ultrasound;protein;extraction;physiochemical property;functional characteristics

期刊名称:POULTRY SCIENCE ( 影响因子:3.352; 五年影响因子:3.679 )

ISSN:

年卷期:

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收录情况: SCI

摘要: This research investigated the physio-chemical and functional characteristics of chicken liver protein isolate, which was extracted by alkali (CLPI) and ultrasound-assisted alkali (UCLPI), respectively. Protein yield of UCLPI was increased by 55.4% over that of CLPI (P < 0.05). Several amino acids were significantly higher and methionine was lower in UCLPI (P < 0.05). Ultrasound treatment reduced the particle size and prolonged the stability of the protein isolate during 7 d storage. Lower pH and a higher amount of total solubilized proteins (68.5%) were found in UCLPI by comparison with CLPI (61.8%). Surface hydrophobicity increased greatly by 63.0% compared with CLPI, but the total/reactive sulfhydryl groups and the primary structure molecular weight profile between them were not significantly different (P > 0.05). However, the enthalpy of denaturation in UCLPI decreased by 41.7%, which was related to the changes in its second structure. This resulted in better water/oil holding capacity and emulsifying properties for UCLPI. The changed physio-chemical characteristics and improved functional properties of UCLPI demonstrated the potential utilization of ultrasound-assisted alkaline extraction in the food industries.

分类号: S8

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