Overexpression of a peanut NAC gene, AhNAC4, confers enhanced drought tolerance in tobacco

文献类型： 外文期刊

第一作者： Tang, G. Y.

作者： Tang, G. Y.;Xu, P. L.;Shan, L.;Shao, F. X.;Liu, Z. J.

作者机构：

关键词： Arachis hypogaea;NAC transcription factor;transgenic tobacco;drought tolerance

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： NAC is one of the most abundant plant-specific groups of transcription factors, which play important roles in plant growth and development regulation, as well as in biotic and abiotic stress responses. In the present study, a stress-responsive NAC gene, AhNAC4, was isolated from a cDNA library of peanut (Arachis hypogaea L.) immature seeds, and characterized for its role in drought tolerance. AhNAC4 shared high amino acid similarity with NAC proteins belonging to the ATAF subfamily. The expression analysis indicated that AhNAC4 was highly induced by drought, salinity and ABA treatments. Transient expression analysis showed the AhNAC4-GFP fusion protein was exclusively localized in the nucleus of onion epidermal cells. Transactivation assays in yeast cells demonstrated that AhNAC4 functioned as a transcription activator and its C-terminus contained the activation domain. Overexpression of AhNAC4 confers enhanced drought tolerance in transgenic tobacco plants. The improved drought tolerance was associated with more stomatal closure and higher water use efficiency. Collectively, our results indicated that AhNAC4 functions as an important regulator in response to drought stress.

分类号： Q94

  • 相关文献

[1]Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton. Wang, Weina,Geng, Shuaipeng,Sun, Quan,Long, Lu,Cai, Chaowei,Liu, Xin,Wang, Guanghao,Miao, Chen,Zhang, Xiao,Cai, Yingfan,Wang, Weina,Yang, Can,Yuan, Youlu,Du, Xiongming,Chu, Zongyan. 2016

[2]SlNAC1, a stress-related transcription factor, is fine-tuned on both the transcriptional and the post-translational level. Kud, Joanna,Niu, Xiangli,Kuhl, Joseph C.,Xiao, Fangming,Huang, Weizao,Liu, Yongsheng,Miao, Min,Niu, Xiangli,Liu, Yongsheng,Ouyang, Bo,Zhang, Junhong,Ye, Zhibiao. 2013

[3]The Novel Wheat Transcription Factor TaNAC47 Enhances Multiple Abiotic Stress Tolerances in Transgenic Plants. Zhang, Lina,Zhang, Lichao,Xia, Chuan,Zhao, Guangyao,Jia, Jizeng,Kong, Xiuying. 2016

[4]Genome-wide analysis of NAM-ATAF1,2-CUC2 transcription factor family in Solanum lycopersicum. Su, Hongyan,Han, Liya,Zhang, Shizhong,Yin, Yanlei,Zhu, Dongzi.

[5]Zea mays NAC transcription factor family members: their genomic characteristics and relationship with drought stress. Li, Liang,Ma, Yiwen,Li, Liang,Ma, Yiwen,Zhang, Shihuang,Hao, Zhuanfang,Li, Xinhai. 2015

[6]Expression of SbSNAC1, a NAC transcription factor from sorghum, confers drought tolerance to transgenic Arabidopsis. Lu, Min,Zhang, Deng-Feng,Shi, Yun-Su,Song, Yan-Chun,Wang, Tian-Yu,Li, Yu,Lu, Min.

[7]Identification of 32 full-length NAC transcription factors in ramie (Boehmeria nivea L. Gaud) and characterization of the expression pattern of these genes. Liu, Touming,Zhu, Siyuan,Tang, Qingming,Tang, Shouwei.

[8]Identification of Expressed Resistance Gene Analogs from Peanut (Arachis hypogaea L.) Expressed Sequence Tags. Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Chen, Xiaoping,Culbreath, Albert K.,Liu, Zhanji,Feng, Suping,Pandey, Manish K.,Varshney, Rajeev K.,Chen, Xiaoping,Guo, Baozhu. 2013

[9]Comparative transcriptome analysis of basal and zygote-located tip regions of peanut ovaries provides insight into the mechanism of light regulation in peanut embryo and pod development. Zhang, Ye,Wang, Pengfei,Xia, Han,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Zhao, Shuzhen,Wang, Xingjun,Zhang, Ye,Gao, Chao,Wang, Xingjun. 2016

[10]Isolation and Characterization of Putative Acetyl-CoA Carboxylases in Arachis hypogaea L.. Li, Meng-Jun,Xia, Han,Zhao, Chuan-Zhi,Li, Ai-Qin,Li, Chang-Sheng,Bi, Yu-Ping,Wan, Shu-Bo,Wang, Xing-Jun. 2010

[11]Development and application of KASP marker for high throughput detection of AhFAD2 mutation in peanut. Zhao, Shuzhen,Li, Aiqin,Li, Changsheng,Xia, Han,Zhao, Chuanzhi,Zhang, Ye,Hou, Lei,Wang, Xingjun. 2017

[12]Isolation of Arachis hypogaea Na+/H+ antiporter and its expression analysis under salt stress. Wan, Shubo,Meng, Jingjing,Guo, Feng,Li, Xinguo,Wan, Shubo,Meng, Jingjing,Guo, Feng,Li, Xinguo,Xing, Jinyi,Wang, Baozhi,Jia, Kunhang,Wan, Shubo,Meng, Jingjing,Guo, Feng,Li, Xinguo. 2011

[13]Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum. Ding, Yu Fei,Wang, Chuan Tang,Tang, Yue Yi,Wang, Xiu Zhen,Wu, Qi,Yu, Hong Tao,Zhang, Jian Cheng,Cui, Feng Gao,Song, Guo Sheng,Yu, Shan Lin,Hu, Dong Qing,Gao, Hua Yuan. 2012

[14]The effect of low water content on seed longevity. Hu, CL,Zhang, YL,Tao, M,Hu, XR,Jiang, CY. 1998

[15]Overexpression of a novel peanut NBS-LRR gene AhRRS5 enhances disease resistance to Ralstonia solanacearum in tobacco. Chen, Hua,Cai, Tiecheng,Deng, Ye,Zhuang, Weijian,Zhang, Chong,Chen, Hua,Cai, Tiecheng,Deng, Ye,Zhuang, Ruirong,Zhang, Ning,Zeng, Yuanhuan,Zheng, Yixiong,Zhuang, Weijian,Zheng, Yixiong,Tang, Ronghua,Pan, Ronglong,Pan, Ronglong. 2017

[16]Phenotypic evaluation of the Chinese mini-mini core collection of peanut (Arachis hypogaea L.) and assessment for resistance to bacterial wilt disease caused by Ralstonia solanacearum. Jiang, Huifang,Ren, Xiaoping,Chen, Yuning,Huang, Li,Zhou, Xiaojing,Huang, Jiaquan,Liao, Boshou,Froenicke, Lutz,Yu, Jiujiang,Guo, Baozhu. 2013

[17]Characterization of a New Strain of Capsicum chlorosis virus from Peanut (Arachis hypogaea L.) in China. Chen, K.,Xu, Z.,Yan, L.,Wang, G..

[18]Applications of xerophytophysiology in plant production - peanut cultivation with the AnM method. Xu, Hui-lian,Xu, Rongyan,Qin, Feifei,Morita, Shigenori,Wang, Jingshan,Wang, Minglun,Qin, Feifei. 2009

[19]Identification and expression analysis of genes responsive to drought stress in peanut. Hou, L.,Liu, W.,Li, Z.,Huang, C.,Fang, X. L.,Wang, Q.,Liu, X.,Hou, L.,Liu, W.,Li, Z.,Huang, C.,Fang, X. L.,Wang, Q.,Liu, X.,Fang, X. L..

[20]An efficient method for total RNA extraction from peanut seeds. Huang, C.,Picimbon, J. F.,Li, H. Q.,Li, Z.,Liu, Q.,Liu, W.,Huang, C.,Picimbon, J. F.,Li, H. Q.,Li, Z.,Liu, Q.,Liu, W..

作者其他论文 更多>>

微信小程序