A sensitive chemiluminescence enzyme immunoassay based on molecularly imprinted polymers solid-phase extraction of parathion

文献类型： 外文期刊

第一作者： Chen, Ge

作者： Chen, Ge;Jin, Maojun;Du, Pengfei;Zhang, Chan;Cui, Xueyan;Zhang, Yudan;She, Yongxin;Shao, Hua;Jin, Fen;Wang, Shanshan;Zheng, Lufei;Wang, Jing

作者机构：

关键词： Chemiluminescence enzyme immunoassay;Matrix effect;Molecularly imprinted polymers;Parathion

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The chemiluminescence enzyme immunoassay (CLEIA) method responds differently to various sample matrices because of the matrix effect. In this work, the CLEIA method was coupled with molecularly imprinted polymers (MIPs) synthesized by precipitation polymerization to study the matrix effect. The sample recoveries ranged from 72.62% to 121.89%, with a relative standard deviation (RSD) of 3.74 -18.14%.The ratio of the sample matrix-matched standard curve slope rate to the solvent standard curve slope was 1.21, 1.12, 1.17, and 0.85 for apple, rice, orange and cabbage in samples pretreated with the mixture of PSA and C-18. However, the ratio of sample (apple, rice, orange, and cabbage) matrix-matched standard-MIPs curve slope rate to the solvent standard curve was 1.05, 0.92, 1.09, and 1.05 in samples pretreated with MIPs, respectively. The results demonstrated that the matrices of the samples greatly interfered with the detection of parathion residues by CLEIA. The MIPs bound specifically to the parathion in the samples and eliminated the matrix interference effect. Therefore, the CLEIA method have successfully applied MIPs in sample pretreatment to eliminate matrix interference effects and provided a new sensitive assay for agro-products. (C) 2017 Elsevier Inc. All rights reserved.

分类号： Q5`Q`O65

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