Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.)

文献类型： 外文期刊

第一作者： Wang, He

作者： Wang, He;Fan, Mingxia;Wang, He;Fan, Mingxia;Zhang, Chunyu;Ma, Wenjuan;Chang, Jing;Huang, Senxin;Lin, Feng;Wang, He;Wang, Guohong;Shi, Lei;Wei, Zhengyi

作者机构：

关键词： pollen-specific promoter;maize;ZmSTK2_USP gene;PCR;GUS activity

期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）

ISSN：

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收录情况： SCI

摘要： ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP- GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.

分类号： Q3`R394

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