A novel GhBEE1-Like gene of cotton causes anther indehiscence in transgenic Arabidopsis under uncontrolled transcription level

文献类型： 外文期刊

第一作者： Eryong Chen;Xiaoqian Wang

作者： Eryong Chen;Xiaoqian Wang;Zhang, Xueyan;Qian Gong;Hamama Islam Butt;Yanli Chen;Chaojun Zhang;Zuoren Yang;Zhixia Wu;Xiaoyang Ge;Xianlong Zhang;Fuguang Li;Xueyan Zhang

作者机构：

关键词： Brassinosteroids;GhBEE1-Like;Anther dehiscence;Cotton;Arabidopsis

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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年卷期：

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收录情况： SCI

摘要： Male-sterile lines are very important for selective breeding, and anther dehiscence defect is an effective way to generate male-sterile lines. Although several bHLH-family proteins in Arabidopsis have been characterized, little is known about the role of bHLH-family proteins in cotton. Here, we isolated a novel bHLH protein from cotton (Gossypium hirsutum), named GhBEE1-Like. Protein domain analysis showed that GhBEE1-Like contained a basic domain and an HLH domain. Subcellular localization analysis revealed that GhBEE1-Like was a nuclear-localized protein. Expression pattern analysis showed GhBEE1-Like was highly expressed in floral organs, and its expression was induced by the active brassinosteroid (BR) substance 24-epi-BL. GhBEE1-Like overexpression in Arabidopsis resulted in two types of transgenic lines, one with normal anther dehiscence and the other with defective anther dehiscence. Semi-qRT-PCR and qRT-PCR analyses revealed that GhBEE1-Like transcript levels acted as a check-point determining how anther dehiscence proceeds in these transgenic lines; regulated transcript levels result in normal anther dehiscence, whereas uncontrolled transcript levels lead to anther in dehiscence. These results suggest that GhBEE1-Like plays an important role via its accumulation in regulating anther dehiscence. Therefore, controlling the level of GhBEE1-Like expression in cotton could be a convenient tool for generating male-sterile lines to use in selective breeding.

分类号： R394

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