A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of Pepper vein yellows virus

文献类型： 外文期刊

第一作者： Luo, Xiangwen

作者： Luo, Xiangwen;Zhang, Deyong;Liu, Yong;Luo, Xiangwen;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Ouyang, Xian;Zhang, Zhanhong;Zhou, Xuguo;Li, Fan

作者机构：

关键词： detection;pepper;Pepper vein yellows virus;PeVYV;RT-LAMP;RT-PCR

期刊名称：CANADIAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.442； 五年影响因子：1.993 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62 degrees C for 45min and the whole reaction took less than 1h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.

分类号： S4`S43`Q945.8

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