An ultrasensitive label-free electrochemiluminescent immunosensor for measuring CrylAb level and genetically modified crops content

文献类型： 外文期刊

第一作者： Gao, Hongfei

作者： Gao, Hongfei;Wen, Luke;Wu, Yuhua;Wu, Gang;Gao, Hongfei;Wen, Luke;Wu, Yuhua;Wu, Gang;Fu, Zhifeng

作者机构：

关键词： Electrochemiluminescence Label-free immunosensor;Carbon nanospheres;Cry protein;Genetically modified crops

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The development of genetically modified (GM) insect-resistant crops has aroused great public concern about the risks on the eco-environment resulting from a release of toxic Cry proteins (such as CrylAb) to the soil. Therefore, it is of crucial importance to measure the Cry proteins level and the GM crops content. Here, we have tested for the first time a method that uses novel carbon nanospheres (CNPs) label-free electrochemiluminescent (ECL) immunosensor for the ultrasensitive quantification of CrylAb and GM crops. In this work, novel CNPs were prepared from printer toner with a very facile approach, and linked with anti-CrylAb antibodies to modify a golden working electrode. The immunoreaction between CrylAb and its antibody formed an immunocomplex on the bioreceptor region of the sensor, which inhibited electron transfer between the electrode surface and the ECL substance, leading to a decrease of ECL response. Under the optimal conditions, the fabricated label-free ECL immunosensor determined CrylAb down to 3.0 pg mL(-1) within a linear range of 0.010-1.0 ng mL(-1), showing significant improvement of sensitivity than that of most previous reports. Meanwhile, the proposed method was successfully applied for GM rice BT63 and GM maize MON810 detections down to 0.010% and 0.020%, respectively. Due to its outstanding advantages such as high sensitivity, ideal selectivity, simple fabrication, rapid detection, and low cost, the developed method can be considered as a powerful and pioneering tool for GM crops detection. Its use can also be extended to other toxin protein sensing in foods.

分类号： Q

  • 相关文献

[1]Tier-1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non-target arthropods. Romeis, Joerg,Wu, Kong-Ming,Peng, Yu-Fa,Romeis, Joerg. 2014

[2]Crystal structure of Bacillus thuringiensis Cry8Ea1: An insecticidal toxin toxic to underground pests, the larvae of Holotrichia parallela. Wei, Lei,Wu, Xiaoai,Rao, Zihe,Guo, Shuyuan,Guo, Shuyuan,Liu, Yanfeng,Xue, Jing,Wu, Hongfu,Song, Fuping,Zhang, Jie,Huang, Dafang. 2009

[3]Broad specificity immunoassay for detection of Bacillus thuringiensis Cry toxins through engineering of a single chain variable fragment with mutagenesis and screening. Zhong, Jianfeng,Hu, Xiaodan,Zhang, Xiao,Liu, Yuan,Xu, Chongxin,Zhang, Cunzheng,Lin, Manman,Liu, Xianjin. 2018

[4]Impacts of nitrogen fertilizer on major insect pests and their predators in transgenic Bt rice lines T2A-1 and T1C-19. Yang, Yajun,Liu, Kai,Xu, Hongxing,Zheng, Xusong,Tian, Junce,Lu, Zhongxian,Liu, Kai,Han, Hailiang,Wang, Guiyue,Zhang, Facheng,Chen, Guihua.

[5]Identification and utilization of cleistogamy gene cl7(t) in rice (Oryza sativa L.). Ni, Da-Hu,Duan, Yong-Bo,Yang, Ya-Chun,Wei, Peng-Cheng,Li, Hao,Song, Feng-Shun,Ni, Jin-Long,Yang, Jian-Bo,Li, Juan,Duan, Yong-Bo,Wei, Peng-Cheng,Li, Hao,Xu, Rong-Fang,Li, Chun-Rong,Liang, Dan-Dan. 2014

[6]Proteomic identification of differentially expressed proteins in Gossypium thurberi inoculated with cotton Verticillium dahliae. Zhao, Fu'an,Fang, Weiping,Xie, Deyi,Zhao, Yuanming,Tang, Zhongjie,Li, Wu,Nie, Lihong,Lv, Shuping,Zhao, Fu'an. 2012

[7]Meeting demands for increased cereal production in China. He, Zhonghu,Xia, Xianchun,Peng, Shaobing,He, Zhonghu,Lumpkin, Thomas Adam. 2014

[8]Construction of a reference plasmid molecule containing eight targets for the detection of genetically modified crops. Wang, Xiumin,Teng, Da,Yang, Yalin,Tian, Fang,Guan, Qingfeng,Wang, Jianhua,Wang, Xiumin,Teng, Da,Yang, Yalin,Tian, Fang,Guan, Qingfeng,Wang, Jianhua.

[9]Use of an artificial diet system to study the toxicity of gut-active insecticidal compounds on larvae of the green lacewing Chrysoperla sinica. Hu, Long,Romeis, Joerg,Wang, Yanan,Han, Lanzhi,Chen, Xiuping,Peng, Yufa,Romeis, Joerg. 2014

[10]Detection of genetically modified crops using multiplex asymmetric polymerase chain reaction and asymmetric hyperbranched rolling circle amplification coupled with reverse dot blot. Wang, Xiumin,Teng, Da,Guan, Qingfeng,Wang, Jianhua,Wang, Xiumin,Teng, Da,Guan, Qingfeng,Wang, Jianhua,Tian, Fang.

作者其他论文 更多>>

微信小程序