Prevalence, serotype diversity, biofilm-forming ability and eradication of Listeria monocytogenes isolated from diverse foods in Shanghai, China

文献类型： 外文期刊

第一作者： Wang, Wenkai

作者： Wang, Wenkai;Zhou, Xiujuan;Cheng, Mengya;Shi, Chunlei;Shi, Xianming;Suo, Yujuan;Deng, Xiangyu

作者机构：

关键词： Listeria monocytogenes;Food isolates;Serotypes;Biofilm;Disinfectants

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Listeria monocytogenes is a major foodborne pathogen that is well known for its high mortality rate in humans upon infection. This study aimed to investigate the prevalence and serotype diversity of L. monocytogenes isolates in various foods. From July 2013 to June 2014, a total of 648 food samples, originating from raw milk, quick-frozen food, vegetables, raw meats, cooked meats, aquatic products, dairy products and bean products, were collected from open-air markets, large supermarkets, delis and dairy farms, in Shanghai, China. Forty samples (6.2%) tested positive for L. monocytogenes, confirmed by both bacteriological and molecular methods. Among the food categories surveyed, the highest prevalence was observed in raw meat samples at 16.0% (15/94), followed by 9.9% (10/101) in quick-frozen food samples and 73% (4/55) in cooked meat samples. Serotyping showed that majority of the isolates (67.5%) belonged to serotype 1/2a, followed by 1/2b (22.5%),112c (7.5%) and 3c (2.5%). Isolates were assessed for their ability to form biofilms. Significant differences in biofilm forming abilities were observed between storage temperatures and serotypes. Anti-biofilm efficacy of industrial disinfectants, chlorine dioxide (ClO2) and hydrogen peroxide (H2O2), was examined by measuring minimal effective concentration (MEC) on L. monocytogenes in biofilm. A positive correlation was observed among the biofilm's resistance to H2O2 and the biomass of biofilms. ClO2 was found to be more effective against biofilm and inactivated all the listerial cells in the biofilm state, whereas H2O2 was not able to kill all the cells in biofilms at the recommended concentrations according to National Food Safety Standard of China. Our study suggests that there is potential public health risk of L. monocytogenes infection in Shanghai. Efficacy against L. monocytogenes biofilm should be considered when evaluating disinfectants. (C) 2016 Published by Elsevier Ltd.

分类号： TS2

  • 相关文献

[1]Occurrence and antimicrobial susceptibility of Listeria monocytogenes isolates from retail raw foods. Wang, Xiu-Mei,Liu, Hui-Fang,Zhang, Wan-Jiang,Si, Wei,Yu, Shen-Ye,Liu, Si-Guo,Lu, Xing-Feng,Yin, Lu,Shao, Mei-Li. 2013

[2]Effects of phenyllactic acid as sanitizing agent for inactivation of Listeria monocytogenes biofilms. Liu, Fang,Du, Lihui,Zhao, Tong,Zhao, Ping,Doyle, Michael P..

[3]Evaluation of aphid transmission abilities and vector transmission phenotypes of barley yellow dwarf viruses in China. Li, L.,Liu, L.,Wang, X. F.,Zhou, G..

[4]Characterization of Streptococcus suis isolates from the diseased pigs in China between 2003 and 2007. Wei, Zigong,Li, Ran,Zhang, Anding,He, Hongkui,Hua, Yafeng,Xia, Jing,Chen, Huanchun,Jin, Meilin,Wei, Zigong,Li, Ran,Zhang, Anding,He, Hongkui,Hua, Yafeng,Xia, Jing,Chen, Huanchun,Jin, Meilin,Cai, Xuehui.

[5]Phylogenetic analysis of the haemagglutinin gene of canine distemper virus strains detected from breeding foxes, raccoon dogs and minks in China. Zhao, Jian-Jun,Yan, Xi-Jun,Chai, Xiu-Li,Luo, Guo-Liang,Zhang, Hai-Ling,Gao, Han,Bai, Xue,Zhang, Lei,Chen, Tao,Xu, Lei,Zhao, Chun-Fei,Wang, Feng-Xue,Shao, Xi-Qun,Wu, Wei,Cheng, Shi-Peng,Martella, Vito,Liu, Ying-Xue.

[6]Screening and identification of B cell epitopes of structural proteins of foot-and-mouth disease virus serotype Asia1. Zhang, Zhong-Wang,Zhang, Yong-Guang,Wang, Yong-Lu,Pan, Li,Fang, Yu-Zhen,Jiang, Shou-Tian,Lue, Jian-Liang,Zhou, Peng.

[7]Rapid Detection of Listeria monocytogenes in Raw Milk with Loop-Mediated Isothermal Amplification and Chemosensor. Wang, Deguo,Zhang, Gaiping,Deng, Ruiguang,Zhi, Aimin,Guo, Junqing,Zhao, Dong,Wang, Deguo,Xu, Zhihong,Lu, Chengping. 2011

[8]RsbV of Listeria monocytogenes contributes to regulation of environmental stress and virulence. Zhang, Zaichao,Meng, Qingling,Qiao, Jun,Yang, Lihong,Wang, Guanglei,Chen, Chuangfu,Meng, Qingling,Cai, Xuepeng,Zhang, Lijuan.

[9]Modelling the cross-contamination of Listeria monocytogenes in pork during bowl chopping. Jiang, Ronghua,Wang, Xiang,Liu, Yangtai,Dong, Qingli,Wang, Wen,Wang, Wen,Du, Jianping,Cui, Yang,Zhang, Chunyan. 2018

[10]Impact of rli87 gene deletion on response of Listeria monocytogenes to environmental stress. Xie Kun,Meng Qingling,Jun Qiao,Peng Yelong,Liu Tianli,Chen Cheng,Ma Yu,Hu Zhengxiang,Chen Chuangfu,Cai Xuepeng.

[11]The roles of noncoding RNA Rli60 in regulating the virulence of Listeria monocytogenes. Peng, Ye-Long,Meng, Qing-Ling,Qiao, Jun,Xie, Kun,Chen, Cheng,Liu, Tian-Li,Hu, Zheng-Xiang,Ma, Yu,Chen, Chuang-Fu,Cai, Xue-Peng.

[12]Inactivation of Salmonella spp. and Listeria spp. by Palmitic, Stearic, and Oleic Acid Sophorolipids and Thiamine Dilauryl Sulfate. Zhang, Xuejie,Ashby, Richard,Solaiman, Daniel K. Y.,Uknalis, Joseph,Fan, Xuetong. 2016

[13]Bacteriocinogenic Enterococcus faecium inhibits the virulence property of Listeria monocytogenes. Ye, Keping,Huang, Yan,Liu, Jia,Liu, Mei,Zhou, Guanghong,Zhang, Xinxiao. 2018

[14]Characterization of Enterococcus durans 152 bacteriocins and their inhibition of Listeria monocytogenes in ham. Du, Lihui,Liu, Fang,Zhao, Ping,Zhao, Tong,Doyle, Michael P..

[15]Plasmid-borne cadmium resistant determinants are associated with the susceptibility of Listeria monocytogenes to bacteriophage. Zhang, Hui,Zhou, Yan,Bao, Hongduo,Zhang, Lili,Wang, Ran,Zhang, Hui,Zhou, Xiaohui.

[16]Whole transcriptomic analysis of the plant-beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 during enhanced biofilm formation regulated by maize root exudates. Zhang, Nan,Yang, Dongqing,Wang, Dandan,Miao, Youzhi,Shao, Jiahui,Zhou, Xuan,Xu, Zhihui,Li, Qing,Feng, Haichao,Li, Shuqing,Shen, Qirong,Zhang, Ruifu,Zhang, Ruifu,Shen, Qirong,Zhang, Ruifu. 2015

[17]Antibacterial and antibiofilm activity of phenyllactic acid against Enterobacter cloacae. Liu, Fang,Wang, Fengting,Wang, Daoying,Zhang, Xinxiao,Sun, Zhilan,Xu, Weimin,Du, Lihui,Zhao, Tong,Doyle, Michael P.. 2018

[18]Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp avenae Strain RS-2. Masum, Md. Mahidul Islam,Yang, Yingzi,Li, Bin,Olaitan, Ogunyemi Solabomi,Chen, Jie,Zhang, Yang,Fang, Yushi,Qiu, Wen,Masum, Md. Mahidul Islam,Wang, Yanli,Sun, Guochang. 2017

[19]Penicillin-resistant characterization of Staphylococcus aureus isolated from bovine mastitis in Gansu, China. Yang Feng,Liu Long-Hai,Wang Ling,Wang Xu-rong,Li Xin-Pu,Jin-yin, Luo,Zhang Zhe,Zhang Shi-dong,Yan Zuo-ting,Li Hong-sheng. 2017

[20]FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp cucumerinum. Li, Peidian,Pu, Xiaoming,Yang, Qiyun,Shen, Huifang,Zhang, Jingxin,Lin, Birun,Li, Peidian,Feng, Baozhen,Li, Peidian. 2015

作者其他论文 更多>>

微信小程序