Characterization of -catenin 1 during the gonad development in the common carp (Cyprinus carpio)

文献类型: 外文期刊

第一作者: Wang, Lan-mei

作者: Wang, Lan-mei;Zhu, Wen-bin;Fu, Jian-jun;Dong, Zai-jie;Song, Fei-biao;Dong, Juan-juan;Dong, Zai-jie

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关键词: 17-ethinylestradiol (E2);cDNA cloning;Cyprinus carpio;expression;localization;-catenin 1

期刊名称:AQUACULTURE RESEARCH ( 影响因子:2.082; 五年影响因子:2.415 )

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收录情况: SCI

摘要: -catenin gene is a pivotal gene for gonad development and maintenance of ovarian function in mammals. However, little is known about its expression and function in gonad development of fish. In this study, a complete cDNA (3342bp) sequence of -catenin 1 was cloned from the common carp, Cyprinus carpio, by RACE PCR, which encodes a 780-amino-acid protein. Quantitative real-time PCR demonstrated that -catenin 1 mRNA expressions were high in the testis and ovary tissue and the expression increased as the testes developed and the early stage ovaries developed. Western blot results revealed a single immunoreactive band with an estimated molecular weight of 90kDa in testes. Immunohistochemistry analysis revealed that the -catenin 1 protein was concentrated mainly in the cytoplasm of early development stage of oocyte cells and in the cytomembrane of developing and mature sperm cells. 17-Ethinylestradiol injecting intraperitoneally into the fish decreased the relative -catenin 1 mRNA expression level except 1g/g 72hr and 5g/g 48hr of treatments in the ovary by real-time PCR. These results suggest, for the first time, that -catenin 1 is an essential protein in gonad development and might be involved in ovarian early development of C.carpio.

分类号: S9

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