Butyric acid regulates progesterone and estradiol secretion via cAMP signaling pathway in porcine granulosa cells

文献类型: 外文期刊

第一作者: Lu, Naisheng

作者: Lu, Naisheng;Li, Mengjiao;Lei, Hulong;Jiang, Xueyuan;Tu, Weilong;Lu, Yang;Xia, Dong

作者机构:

关键词: Butyric acid;Porcine granulosa cells;Progesterone;Estradiol;cAMP signaling pathway

期刊名称:JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY ( 影响因子:4.292; 五年影响因子:4.216 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Butyric acid (BA), one of the short chain fatty acids (SCFAs), has positive actions on the metabolism, inflammation, etc. However, whether it influences the reproductive physiology and if so the detail mechanism involved has not yet been determined. In this study, the porcine granulosa cells (PGCs) were treated with gradient concentrations of BA. After 24 h culture, 0.05 mM BA significantly stimulated the progesterone (P-4) secretion (P < 0.05), 5 mM and 10 mM BA significantly inhibited the P-4 secretion (P < 0.05). Simultaneously, BA up-regulated the estradiol (E-2) secretion in a dose dependent manner, 5 mM and 10 mM BA significantly promoted the E-2 level (P < 0.05). In addition, 10 mM BA significantly promoted the G-protein-coupled receptor 41/43 mRNA (P < 0.05). Interestingly, 5 mM BA treatment significantly down-regulated cyclic adenosine monophosphate (cAMP) content (P < 0.05), steroidogenic acute regulatory (StAR), steroidogenic factor 1 (SF1), P450scc in the mRNA and/or protein level (P < 0.05), and these actions were reversed by cAMP activator forskolin (FK). Moreover, the co-treatment of 5 mM BA and bupivacaine (BPC, the cAMP inhibitor) significantly accumulated the inhibition action of BPC on cAMP, the secretion of P-4, and the abundance of StAR mRNA (P < 0.05), inhibited the up-regulation of 5 mM BA on the E-2 secretion (P < 0.05). Further, the Global Proteome and KEGG pathway analysis found that 5 mM BA significantly up-regulated the I3LM80 proteins (P < 0.05), which is involved in the steroid biosynthesis signaling pathway. 5 mM BA significantly decreased the F2Z5G3 protein level (P < 0.05), and the cAMP signaling pathway. In conclusion, present findings for the first time demonstrated that BA could regulate the P-4 and E-2 hormone synthesis in PGCs via the cAMP signaling pathway.

分类号: Q5

  • 相关文献

[1]Effect of SIRT1 on cellular apoptosis and steroidogenesis in bovine ovarian granulosa cells in vitro. Tan, X. W.,You, W.,Song, E. L.,Zhao, H. B.,Liu, X. M.,Wang, H. Z.,Liu, G. F.,Cheng, H. J.,Liu, Y. F.,Wan, F. C.,Tan, X. W.,You, W.,Song, E. L.,Zhao, H. B.,Liu, X. M.,Wang, H. Z.,Liu, G. F.,Cheng, H. J.,Liu, Y. F.,Wan, F. C..

[2]miR-15b negatively correlates with lipid metabolism in mammary epithelial cells. Chu, Meiqiang,Zhao, Yong,Yu, Shuai,Hao, Yanan,Zhang, Pengfei,Feng, Yanni,Ma, Dongxue,Li, Lan,Shen, Wei,Min, Lingjiang,Zhang, Hongfu,Liu, Jing,Cheng, Ming,Cao, Hongfang,Li, Qiang. 2018

[3]MicroRNA-126 participates in lipid metabolism in mammary epithelial cells. Chu, Meiqiang,Zhao, Yong,Feng, Yanni,Li, Lan,Shen, Wei,Min, Lingjiang,Zhang, Hongfu,Liu, Jing,Cheng, Ming,Cao, Hongfang,Li, Qiang. 2017

[4]Mammalian steroid hormones can reduce abundance and affect the sex ratio in a soil nematode community. Hu, Cheng,Hermann, Galit,Pen-Mouratov, Stanislav,Steinberger, Yosef,Hu, Cheng,Shore, Laurence. 2011

[5]p53 and NF kappa B regulate microRNA-34c expression in porcine ovarian granulosa cells. Xu Yuan,Xiao Guang,Zhang Zhe,Chen Zan-mou,Zhang Hao,Li Jia-qi,Zhang Ai-ling. 2016

[6]Effect of BMPRIB gene silencing by siRNA on apoptosis and steroidogenesis of porcine granulosa cells. Zhao, Y. Y.,Li, X. X.,Chen, X.,Yu, P.,Wang, J. J.,Xu, Y. X.,Wang, W.. 2014

[7]Molecular mechanisms of enhancing porcine granulosa cell proliferation and function by treatment in vitro with anti-inhibin alpha subunit antibody. Cai, Liuping,Sun, Aidong,Li, Hui,Yu, Jianning,Ying, Shijia,Chen, Zhe,Shi, Zhendan,Tsinkgou, Anastasia. 2015

[8]Physiological responses and gene expression changes in the western mosquitofish (Gambusia affinis) exposed to progesterone at environmentally relevant concentrations. Hou, Liping,Yang, Yang,Shu, Hu,Cheng, Xuemei,Xu, Hongyan,Ying, Guangguo,Zhao, Jianliang,Ying, Guangguo,Zhao, Jianliang. 2017

[9]Fecal progestin concentrations as an indicator of reproductive success in American Mink. Cao, Xinyan,Wei, Haijun,Xue, Hailong,Li, Xiaoxia,Zhao, Weigang,Xu, Chao,Wang, Shiyong,Diao, Yunfei,Xu, Baozeng,Cao, Xinyan,Wei, Haijun,Xue, Hailong,Li, Xiaoxia,Zhao, Weigang,Xu, Chao,Wang, Shiyong,Diao, Yunfei,Xu, Baozeng,Rose, Jack.

[10]A Survey of Naturally-Occurring Steroid Hormones in Raw Milk and the Associated Health Risks in Tangshan City, Hebei Province, China. Qu, Xueyin,Su, Chuanyou,Zheng, Nan,Li, Songli,Meng, Lu,Wang, Jiaqi,Qu, Xueyin,Su, Chuanyou,Zheng, Nan,Li, Songli,Meng, Lu,Wang, Jiaqi,Qu, Xueyin. 2018

[11]Diurnal variations of progesterone, testosterone, and androsta-1,4-diene-3,17-dionein the rumen and in vitro progesterone transformation by mixed rumen microorganisms of lactating dairy cows. Ren, Qing-Chang,Yang, Hong-Jian,Li, Sheng-Li,Wang, Jia-Qi. 2014

[12]Inhibition of peripubertal sheep mammary gland development by cysteamine through reducing progesterone and growth factor production. Zhao, Yong,Liu, Xinqi,Zhang, Pengfei,Zhao, Yong,Feng, Yanni,Li, Lan,Zhang, Pengfei,Cui, Liantao,Chu, Meiqiang,Shen, Wei,Feng, Yanni,Li, Lan,Cui, Liantao,Chu, Meiqiang,Shen, Wei,Min, Lingjiang,Zhang, Hongfu,Kou, Xin.

[13]Dietary -arginine supplementation enhances placental growth and reproductive performance in sows. Gao, Kaiguo,Jiang, Zongyong,Lin, Yingcai,Zheng, Chuntian,Zhou, Guilian,Chen, Fang,Gao, Kaiguo,Yang, Lin,Wu, Guoyao. 2012

[14]Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages. Liao, T.,Guo, Q. L.,Xu, Y.,Liao, T.,Cheng, W.,Jin, S. W.. 2009

[15]Bidirectional Estrogen-Like Effects of Genistein on Murine Experimental Autoimmune Ovarian Disease. Ding, Qiao,Wang, Yuxiao,Li, Na,Hu, Jielun,Nie, Shaoping,Zhu, Kexue,Wang, Sunan,Zhu, Fan. 2016

[16]Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples. Yang, Xing-dong,Wang, Fang-yu,Zhang, Gai-ping,Yang, Xing-dong,Zeng, Xian-yin,Song, Chun-mei,Wu, Shu-yan,Zhang, Gai-ping.

[17]Lipopolysaccharide and heat stress impair the estradiol biosynthesis in granulosa cells via increase of HSP70 and inhibition of smad3 phosphorylation and nuclear translocation. Li, Hui,Cai, Liuping,Shi, Zhendan,Guo, Shuangshuang,Ma, Weiming.

作者其他论文 更多>>