Effect of protein restriction followed by realimentation on growth, nutrient digestibility, ruminal parameters, and transporter gene expression in lambs

文献类型： 外文期刊

第一作者： Ma, T.

作者： Ma, T.;Wang, B.;Zhang, N.;Tu, Y.;Si, B.;Cui, K.;Qi, M.;Diao, Q.

作者机构：

关键词： Protein restriction and realimentation;Rumen epithelium;Gene expression;Volatile fatty acid

期刊名称：ANIMAL FEED SCIENCE AND TECHNOLOGY （ 影响因子：3.247； 五年影响因子：3.806 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We report the effects of a protein-restricted diet and realimentation with a milk replacer (MR) and starter on the growth, digestibility, fermentation, and expression of the genes involved in volatile fatty acid (VFA) absorption in the lamb ruminal epithelium. Sixteen newborn twin Hu lamb pairs were used. Each pair was fed the MR and starter with a normal protein level (NPL, 250 and 210 g/kg, respectively) or a low protein level (LPL, 190 and 150 g/kg, respectively) from 15 to 60 days of age (restriction period). Then, all lambs were fed the starter containing 210 g crude protein/kg diet from 61 to 90 days of age (realimentation period). Intake was measured daily, and body weight was measured at 15, 60, and 90 days of age. Digestibility trials were carried out from 51 to 60 and from 81 to 90 days of age with four lambs randomly selected from each group. Eight lambs from each group were slaughtered on 60 and 90 days of age before morning feeding, and their ruminal fermentation parameters were measured. The expression levels of the genes involved in VFA absorption in ruminal epitheliums were measured. During the restriction period, feed intake did not differ between the groups (P = 0.116). The final body weight (P = 0.006) and the average daily gain (ADG; P = 0.003) was greater, while the feed conversion ratio (P = 0.011) was lower in the NPL group. No difference was observed in dry matter (P = 0.894) and organic matter (P = 0.495) digestibility, or the N retention/N intake (P = 0.607). The ruminal pH of the lambs fed the LPL diets was greater (P = 0.012). A decrease in ammonia content (P = 0.023) and the total VFA concentration (P < 0.001) was found in the LPL lambs. The molecular acetate content was greater in the LPL group (P = 0.002), but that of propionate was lower (P = 0.034). Protein restriction decreased the mRNA abundance of the transporters, anion exchanger 2 (P = 0.019) and was downregulated in adenoma (P = 0.025), Na+/H+ exchanger 1 (P = 0.017), 2 (P = 0.025), and 3 (P = 0.037). After realimentation, body weight (P = 0.006) and ADG (P = 0.013) were lower in the lambs fed the LPL diet. The feed conversion ratio (P = 0.301) was the same in the two groups. Lambs fed a LPL diet had a greater N retention/N intake (P = 0.031). No difference was determined in the ruminal pH or the total VFA concentration between the groups (P = 0.572). A greater mRNA level of monocarboxylic acid transporter 4 (P = 0.021) and Na+/H+ exchanger 2 (P = 0.049) was observed in the LPL group. These results suggest that realimentation partially recovers nutrient requirements and ruminal function, but lambs subjected to protein restriction still physically lag behind those continuously fed the NPL diet, indicating a permanent effect on later growth owing to nutrient restriction during very early life.

分类号： S2

  • 相关文献

[1]Promotion and Inhibition of Ruminal Epithelium Growth by Butyric Acid and Insulin-Like Growth Factor-1 (IGF-1) in Dairy Goats. Liu Da-cheng,Zhou Xiang-li,Liu Guo-juan,Liu Da-cheng,Zhou Xiang-li,Liu Guo-juan,Gao Min,Hu Hong-lian. 2014

[2]Removal of early fruiting branches impacts leaf senescence and yield by altering the sink/source ratio of field-grown cotton. Chen, Yizhen,Dong, Hezhong,Chen, Yizhen,Kong, Xiangqiang,Dong, Hezhong,Kong, Xiangqiang,Dong, Hezhong. 2018

[3]Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803. Chen, Gao,Bian, Fei,Peng, Zhenying,Zhang, Yan,Yu, Jinhui,Xuan, Ning,Bi, Yuping,He, Qingfang,Chen, Gao,Bian, Fei,Peng, Zhenying,Zhang, Yan,Yu, Jinhui,Xuan, Ning,Bi, Yuping,He, Qingfang,He, Qingfang,Wang, Qiang,Qu, Shujie,Ge, Haitao. 2014

[4]Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803. Chen, Gao,Zhang, Yan,Peng, Zhenying,Fan, Zhongxue,Bian, Fei,Yu, Jinhui,Chen, Gao,Zhang, Yan,Peng, Zhenying,Fan, Zhongxue,Bian, Fei,Yu, Jinhui,Chen, Jun,Qin, Song,Chen, Jun,He, Qingfang. 2017

[5]Transcriptome Analysis of Sucrose Metabolism during Bulb Swelling and Development in Onion (Allium cepa L.). Zhang, Chunsha,Zhang, Hongwei,Liang, Yi,Zhan, Zongxiang,Liu, Bingjiang,Chen, Zhentai. 2016

[6]Analysis of Differentially Expressed Genes in Genic Male Sterility Cotton (Gossypium hirsutum L.) Using cDNA-AFLP. Xiaoding Ma,Chaozhu Xing,Liping Guo,Yangcang Gong,Hailin Wang,Yunlei Zhao,Jianyong Wu. 2007

[7]Exogenous nitric oxide delays salt-induced leaf senescence in cotton (Gossypium hirsutum L.). Kong, Xiangqiang,Wang, Tao,Li, Weijiang,Tang, Wei,Zhang, Dongmei,Dong, Hezhong,Wang, Tao,Dong, Hezhong.

[8]Variants and Gene Expression of the TLR2 Gene and Susceptibility to Mastitis in Cattle. Huang, Jinming,Liu, Li,Wang, Hongmei,Zhang, Cuixia,Ju, Zhihua,Wang, Changfa,Zhong, Jifeng.

[9]Genome-Wide Analysis of Gene Expression Provides New Insights into Cold Responses in Thellungiella salsuginea. Wang, Jiangshan,Cui, Feng,Hou, Lei,Zhao, Shuzhen,Xia, Han,Qiu, Jingjing,Li, Tingting,Zhang, Ye,Wang, Xingjun,Zhao, Chuanzhi,Wang, Jiangshan,Zhang, Quan,Qiu, Jingjing,Wang, Xingjun,Zhao, Chuanzhi. 2017

[10]Differential Gene Expression Between Hybrids and Their Parents During the Four Crucial Stages of Cotton Growth and Development. Zhao Yun-lei,Yu Shu-xun,Xing Chao-zhu,Fan Shu-li,Song Mei-zhen,Ye Wu-wei. 2009

[11]Isolation and expression profiling of GhNAC transcription factor genes in cotton (Gossypium hirsutum L.) during leaf senescence and in response to stresses. Syed Tariq Shah,Chaoyou Pang,Shuli Fan,Meizhen Song,Saima Arain,Shuxun Yu.

[12]Isolation and Characterization of an ERF Transcription Factor Gene from Cotton (Gossypium barbadense L.). Xianpeng Meng,Fuguang Li,Chuanliang Liu,Chaojun Zhang,Zhixia Wu,Yajuan Chen.

[13]Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development. Jiao, Yun,Ma, Rui-juan,Shen, Zhi-jun,Yan, Juan,Yu, Ming-liang. 2014

[14]Response of the enzymes to nitrogen applications in cotton fiber (Gossypium hirsutum L.) and their relationships with fiber strength. Wang YouHua,Feng Ying,Xu NaiYin,Chen BingLin,Ma RongHui,Zhou ZhiGuo,Xu NaiYin. 2009

[15]Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real-time PCR. Fang, Peng,Sun, Feng,Lan, Ying,Du, Linlin,Zhou, Yijun,Zhou, Tong,Fang, Peng,Sun, Feng,Lan, Ying,Du, Linlin,Zhou, Yijun,Zhou, Tong,Fang, Peng,Lu, Rongfei,Shen, Wenbiao. 2015

[16]Single nucleotide polymorphism scanning and expression of the FRZB gene in pig populations. Wang, Zhixiu,Zhang, Bo,Yang, Yuzeng,Ban, Dongmei,Zhang, Hao,Li, Qinggang,Lu, Yunfeng. 2014

[17]Functional characteristic and differential expression of myostatin in Chlamys nobilis. Zhu, Kecheng,Guo, Huayang,Zhang, Nan,Li, Yundong,Jiang, Shigui,Zhang, Dianchang. 2017

[18]Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit. Qin Gai-hua,Qi Yong-jie,Gao Zheng-hui,Pan Hai-fa,Xu Yi-liu,Qin Gai-hua,Qi Xiao-xiao,Qi Yong-jie,Gao Zheng-hui,Pan Hai-fa,Xu Yi-liu,Qi Xiao-xiao. 2017

[19]Gene expression profiling of rootstock '140Ru' and Vitis vinifera L. cv. 'Crimson Seedless' grape roots infected with grape phylloxera. Du, Yuan-Peng,Wang, Feng-Pan,Zhang, Shi-Zhong,Zhai, Heng,Jiang, En-Shun. 2014

[20]Isolation and expression analysis of four HD-ZIP III family genes targeted by microRNA166 in peach. Zhang, C. H.,Zhang, B. B.,Ma, R. J.,Yu, M. L.,Guo, S. L.,Guo, L.. 2015

作者其他论文 更多>>

微信小程序