Development and application of an allele-specific PCR assay for detecting T409C mutation of cyp51 gene linked with tebuconazole resistance in Villosiclava virens (rice false smut)

文献类型： 外文期刊

第一作者： Shi, Niu-Niu

作者： Shi, Niu-Niu;Ruan, Hong-Chun;Chen, Fu-Ru;Yang, Xiu-Juan;Dai, Yu-Li;Gan, Lin;Du, Yi-Xin;Zhang, Yu-Long

作者机构：

关键词： allele-specific polymerase;cyp51;resistance;rice false smut;tebuconazole;Villosiclava virens

期刊名称：CANADIAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.442； 五年影响因子：1.993 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Villosiclava virens is an ascomycete fungus (anamorph: Ustilaginoidea virens) recognized as one of the most economically important pathogens on rice in China that causes false smut disease. In our study, the V. virens cyp51 gene encoding the target protein for fungicide resistant to tebuconazole was cloned. Sequencing showed that fungal mutant strains resistant to tebuconazole harboured the T409C mutation in cyp51. A simple and rapid allele-specific PCR assay was developed to detect the T409C mutation. Among the isolates tested, a unique 391-bp fragment was amplified from resistant mutant strains with primer pair Uv137F1/Uv137DR, whereas no product was amplified from all sensitive isolates. Our study demonstrated that this allele-specific PCR assay is a rapid, simple and reliable method for identification of V. virens mutants resistant to tebuconazole.

分类号： Q95

  • 相关文献

[1]Bioactive Bis-naphtho-gamma-pyrones from Rice False Smut Pathogen Ustilaginoidea virens. Lu, Shiqiong,Sun, Weibo,Meng, Jiajia,Wang, Ali,Wang, Xiaohan,Tian, Jin,Fu, Xiaoxiang,Lai, Daowan,Zhou, Ligang,Dai, Jungui,Dai, Jungui,Liu, Yang.

[2]Development of a Monoclonal Antibody-Based icELISA for the Detection of Ustiloxin B in Rice False Smut Balls and Rice Grains. Fu, Xiaoxiang,Wang, Ali,Wang, Xiaohan,Lin, Fengke,He, Lishan,Lai, Daowan,Zhou, Ligang,Wang, Baoming,Liu, Yang,Li, Qing X.. 2015

[3]A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples. Fu, Xiaoxiang,Wang, Xiaohan,Cui, Yongliang,Wang, Ali,Lai, Daowan,Wang, Baomin,Zhou, Ligang,Liu, Yang,Li, Qing X..

[4]Analysis of the Resistance to Rice Blast and False Smut of 18 Varieties of Hybrid Rice in Sichuan Province, China. Shi, Jun,Xiang, Zufen,Shi, Shoupei,Peng, Tao,Liu, Dingyou,Huang, Tingyou,Hu, Rongping. 2017

[5]Differential expression profiling of the early response to Ustilaginoidea virens between false smut resistant and susceptible rice varieties. Han, Yanqing,Zhang, Kang,Yang, Jun,Zhang, Nan,Fang, Anfei,Zhang, Yong,Sun, Wenxian,Han, Yanqing,Zhang, Kang,Yang, Jun,Zhang, Nan,Fang, Anfei,Zhang, Yong,Sun, Wenxian,Liu, Yongfeng,Chen, Zhiyi,Hsiang, Tom. 2015

[6]The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides. Chen, F.,Diao, Y.,Liu, X.,Fan, J.,Chen, F.,Cools, H. J.,Kelly, S. L.,Kelly, S. L.. 2014

[7]Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR. Li, H.,Ni, D. H.,Li, J.,Li, H.,Ni, D. H.,Duan, Y. B.,Li, J.,Song, F. S.,Li, L.,Wei, P. C.,Yang, J. B.,Li, H.,Wei, P. C.,Duan, Y. B.,Song, F. S.,Chen, Y.. 2013

[8]The role of Ustilaginoidea virens sclerotia in increasing incidence of rice false smut disease in the subtropical zone in China. Yong, Mingli,Deng, Qide,Fan, Linlin,Wang, Zhengyi,Hu, Dongwei,Miao, Jiankun,Wang, Shu,Bai, Yuanjun,Lai, Chaohui,Chen, Hongming,Yang, Xiujuan,Chen, Furu,Jin, Li,Yang, Binghui. 2018

[9]Epiphytic colonization of Ustilaginoidea virens on biotic and abiotic surfaces implies the widespread presence of primary inoculum for rice false smut disease. Guo, X. -Y.,Li, Y.,Li, L.,Xu, Y. -J.,Zhao, J. -Q.,Wang, W.,Guo, X. -Y.,Xiong, H.,Huang, F.,Liu, Y. -F.,Yu, J. -J..

[10]Identification of pathogenicity-related genes in the rice pathogen Ustilaginoidea virens through random insertional mutagenesis. Yu, Mina,Yu, Junjie,Hu, Jiankun,Huang, Lei,Wang, Yahui,Yin, Xiaole,Nie, Yafeng,Meng, Xiangkun,Wang, Weiduo,Liu, Yongfeng,Hu, Jiankun,Huang, Lei.

[11]Transcriptional profiling of the responses to infection by the false smut fungus Ustilaginoidea virens in resistant and susceptible rice varieties. Yang, Chao,Li, Luoye,Li, Jianxiong,Feng, Aiqing,Zhu, Xiaoyuan.

[12]SPECIFIC AND SENSITIVE DETECTION OF THE FUNGAL PATHOGEN USTILAGINOIDEA VIRENS BY NESTED PCR. Zhou Yongli. 2004

[13]Frequency distribution of sensitivity of Ustilaginoidea virens to four EBI fungicides, prochloraz, difenoconazole, propiconazole and tebuconazole, and their efficacy in controlling rice false smut in Anhui Province of China. Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun,Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun,Yao, Jian,Li, Yun-Fei,Chen, Yu,Zhang, Yong,Yang, Xue,Wang, Wen-Xiang,Zhang, Ai-Fang,Gao, Tong-Chun. 2013

[14]Ustiloxin G, a New Cyclopeptide Mycotoxin from Rice False Smut Balls. Wang, Xiaohan,Wang, Jian,Lai, Daowan,Wang, Weixuan,Zhou, Ligang,Dai, Jungui,Dai, Jungui,Liu, Yang. 2017

[15]Determination and Analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in False Smut Balls of Rice. Shan, Tijiang,Sun, Weibo,Liu, Hao,Gao, Shan,Lu, Shiqiong,Wang, Mingan,Sun, Wenxian,Zhou, Ligang,Chen, Zhiyi,Wang, Shu. 2012

[16]Preparative Separation of Main Ustilaginoidins from Rice False Smut Balls by High-Speed Counter-Current Chromatography. Sun, Weibo,Dong, Xuejiao,Xu, Dan,Meng, Jiajia,Fu, Xiaoxiang,Wang, Xiaohan,Lai, Daowan,Zhou, Ligang,Liu, Yang. 2016

[17]Main Ustilaginoidins and Their Distribution in Rice False Smut Balls. Meng, Jiajia,Sun, Weibo,Mao, Ziling,Xu, Dan,Wang, Xiaohan,Lu, Shiqiong,Lai, Daowan,Zhou, Ligang,Zhang, Guozhen,Liu, Yang. 2015

[18]New Ustilaginoidins from Rice False Smut Balls Caused by Villosiclava virens and Their Phytotoxic and Cytotoxic Activities. Sun, Weibo,Wang, Ali,Xu, Dan,Wang, Weixuan,Meng, Jiajia,Lai, Daowan,Zhou, Ligang,Dai, Jungui,Dai, Jungui,Liu, Yang.

[19]Characterization of mating-type loci in rice false smut fungus Villosiclava virens. Yu, Jun-jie,Yu, Mi-na,Yin, Xiao-le,Meng, Xiang-kun,Zhao, Jie,Huang, Lei,Liu, Yong-feng,Sun, Wen-xian,Huang, Li. 2015

[20]The Contents of Ustiloxins A and B along with Their Distribution in Rice False Smut Balls. Wang, Xiaohan,Fu, Xiaoxiang,Lin, Fengke,Sun, Weibo,Meng, Jiajia,Wang, Ali,Lai, Daowan,Zhou, Ligang,Liu, Yang. 2016

作者其他论文 更多>>

微信小程序