Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus

文献类型： 外文期刊

第一作者： Yang, Yang

作者： Yang, Yang;Qin, Xiaodong;Zhang, Wei;Li, Zhiyong;Zhang, Shuaijun;Li, Yanmin;Zhang, Zhidong

作者机构：

关键词： Recombinase polymerase amplification;Real-time RPA assay;RPA LFD assay;PRV

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

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收录情况： SCI

摘要： Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 degrees C within 20 min. The sensitivity of the real-time RPA assay and the RPA LFD assay was 100 copies per reaction and 160 copies per reaction, respectively. Both assays did not detect DNAs from other virus or PRV negative samples. Therefore, the developed RPA assays provide a rapid, simple, sensitive and specific alternative tool for detection of PRV. (C) 2017 Published by Elsevier Ltd.

分类号： R44

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