Isolation, identification and pathogenicity of Vibrio harveyi, the causal agent of skin ulcer disease in juvenile hybrid groupers Epinephelus fuscoguttatus x Epinephelus lanceolatus

文献类型： 外文期刊

第一作者： Shen, G. M.

作者： Shen, G. M.;Shi, C. Y.;Fan, C.;Jia, D.;Wang, S. Q.;Xie, G. S.;Li, G. Y.;Mo, Z. L.;Huang, J.;Shen, G. M.;Shi, C. Y.;Fan, C.;Jia, D.;Wang, S. Q.;Xie, G. S.;Li, G. Y.;Mo, Z. L.;Huang, J.;Shen, G. M.;Fan, C.;Jia, D.

作者机构：

关键词： antibiotic susceptibility;grouper;identification;pathogenicity;Vibrio harveyi

期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.767； 五年影响因子：2.689 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The hybrid grouper, Epinephelus fuscoguttatus (female) x Epinephelus lanceolatus (male), is a newly bred cultivated marine fish species of high economic value. However, a skin ulcer disease with high mortality has occurred, and the responsible pathogen remains unknown. In this study, we summarized the epidemic status and external signs of this disease. We screened potential pathogens and finally isolated one bacterial strain ML01 from affected fish. We subjected healthy juvenile hybrid groupers to bacterial challenge tests with the isolate by immersion, immersion after dermal abrasion and intraperitoneal injection, respectively. Within 14 days post-infection, the isolate ML01 caused mass mortality of juveniles infected via immersion after dermal abrasion or intraperitoneal injection. Diseased juveniles displayed obvioussigns of skin ulcers. The median lethal dose of ML01 by intraperitoneal injection was 1.10 x 10(5) colony-forming units. ML01 was identified as Vibrio harveyi by bacterial morphology, analytical profile index identification, 16S rDNA sequencing and multilocus sequence analysis. Antibiotic susceptibility tests showed that ML01 was sensitive to ceftriaxone, doxycycline and minocycline. The results of this study suggest that V.harveyi is the causal agent of skin ulcer disease in juvenile hybrid groupers, thus providing a basis for effective control and prevention of this disease.

分类号： TS2

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