DEVELOPMENT OF A SPECIES-SPECIFIC REAL-TIME PCR ASSAY FOR DIAGNOSING PANTOEA BEIJINGENSIS

文献类型： 外文期刊

第一作者： Ma, Y.

作者： Ma, Y.;Rong, C.;Wang, S.;Liu, Y.;Wang, J.;Xu, F.;Ma, Y.

作者机构：

关键词： detection;enrichment;hrpK;Pleurotus eryngii;soft rot disease

期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Recently, Pantoea beijingensis has become an emerging pathogen against Pleurotus eryngii, causing soft rot disease in China and Korea. A qPCR assay targeting hrpK, which is predicted to be one of the key genes for the pathogenicity and virulence of P. beijingensis, was developed and evaluated. Approximately 0.1 fg of purified plasmid or 3.23 x 10(2) copies mu l(-1) in pure cultures could be detected. Moreover, the use of an enrichment procedure could induce 100-fold enhancement of the sensitivity. The qPCR assay developed here is a rapid, reliable and sensitive tool for the detection of P. beijingensis at an early stage of infection and it will be useful in the development of management practices.

分类号： Q94

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