An isoflavonoid-enriched extract from Pueraria lobata (kudzu) root protects human umbilical vein endothelial cells against oxidative stress induced apoptosis

文献类型： 外文期刊

第一作者： Gao, Ying

作者： Gao, Ying;Gao, Ying;Wang, Xu;He, Chunnian

作者机构：

关键词： Atherosclerosis;Pueraria lobata;Isoflavonoids;HUVECs;Oxidative stress;Apoptosis

期刊名称：JOURNAL OF ETHNOPHARMACOLOGY （ 影响因子：4.36； 五年影响因子：4.488 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Ethnopharmacological relevance: Reactive oxygen species (ROS) mediate vascular cell dysfunction and lead to atherosclerosis and other chronic cardiovascular diseases. The root of Pueraria lobata (Willd.) Ohwi, also known as kudzu or Gegen (Chinese name), is one of the most important herbs in traditional Chinese medicine and has been widely used in the treatment of cardiovascular diseases, diabetes, osteonecrosis and neurode-gradation diseases. In this study, an ethanol extract from kudzu root was prepared and the in vitro protective effect of the kudzu root extract (KUD) on human umbilical vein endothelial cells (HUVECs) was investigated. Material and methods: An ethanol extract of dried kudzu root was purified with an AB-8 resin column, and the concentrations of puerarin, daidzin and daidzein in the KUD were determined using UV spectroscopy. HUVECs were pretreated with various concentrations of the KUD with or without rotenone and the viability was assessed by AlamarBlue cell viability assay. Next, HUVECs were pretreated with the KUD and then treated with rotenone, and the levels of ROS generation, apoptosis, and changes of the mitochondrial membrane potential (DELTA PSIm) in HUVECs were measured using fluorescent staining assay and high-content analysis. Results: The contents of three major isoflavonoids (puerarin, daidzin and daidzein) were enriched by 7.75-27.51 fold in the extract. The KUD enhanced the proliferation of HUVECs, and protected HUVECs against rotenone-induced oxidative stress and apoptosis. Additionally, the KUD prevented the loss of DELTA PSIm in HUVECs stimulated by oxidative stress. Conclusion: We demonstrated that an isofiavonoid-rich extract prepared from kudzu root has the potential to act as a protector for vascular endothelial cells against intracellular ROS mediated apoptosis and mitochondrial damage.

分类号： R96

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