Endonuclease IV based competitive DNA probe assay for differentiation of low-abundance point mutations by discriminating stable single-base mismatches

文献类型： 外文期刊

第一作者： Xu, Jiaju

作者： Xu, Jiaju;Chen, Na;Liu, Na;Xiao, Xianjin;Li, Longjie;She, Yongxin;Wang, Shanshan

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期刊名称：CHEMICAL COMMUNICATIONS （ 影响因子：6.222； 五年影响因子：6.008 ）

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收录情况： SCI

摘要： We disclosed the unique discrimination property of Endo IV toward stable single-base mismatches located at the second nucleotide 30 to the AP site. Coupled with thermodynamic differentiation and competitive blocker strands, a highly sensitive and specific detection system was established with discrimination factors of 510-1079 for G:X mismatches and LODs of 0.003-0.005% for KRAS G12A, KRAS G12V and KRAS G12S mutations.

分类号： O6

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