Holotrichia oblita Midgut Proteins That Bind to Bacillus thuringiensis Cry8-Like Toxin and Assembly of the H-oblita Midgut Tissue Transcriptome

文献类型： 外文期刊

第一作者： Huang, Ying

作者： Huang, Ying;Shu, Changlong;Song, Fuping;Zhang, Jie;Jiang, Jian;Lai, Jinsheng;Jiang, Jian;Lai, Jinsheng;Soberon, Mario;Bravo, Alejandra;Liu, Chunqing

作者机构：

关键词： Bacillus thuringiensis;Cry8-like;Holotrichia oblita;binding proteins

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The Bacillus thuringiensis strain HBF-18 (CGMCC 2070), containing two cry genes (cry8-like and cry8Ga), is toxic to Holotrichia oblita larvae. Both Cry8-like and Cry8Ga proteins are active against this insect pest, and Cry8-like is more toxic. To analyze the characteristics of the binding of Cry8-like and Cry8Ga proteins to brush border membrane vesicles (BBMVs) in H. oblita larvae, binding assays were conducted with a fluorescent DyLight488-labeled Cry8-like toxin. The results of saturation binding assays demonstrated that Cry8-like bound specifically to binding sites on BBMVs from H. oblita, and heterologous competition assays revealed that Cry8Ga shared binding sites with Cry8-like. Furthermore, Cry8-like-binding proteins in the midgut from H. oblita larvae were identified by pulldown assays and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition, the H. oblita midgut transcrip-tome was assembled by high-throughput RNA sequencing and used for identification of Cry8-like-binding proteins. Eight Cry8-like-binding proteins were obtained from pulldown assays conducted with BBMVs. The LC-MS/MS data for these proteins were successfully matched with the H. oblita transcriptome, and BLASTX results identified five proteins as serine protease, transferrin-like, uncharacterized protein LOC658236 of Tribolium castaneum, ATPase catalytic subunit, and actin. These identified Cry8-like-binding proteins were different from those confirmed previously as receptors for Cry1A proteins in lepidopteran insect species, such as aminopeptidase, alkaline phosphatase, and cadherin.

分类号： Q939.9`X1

  • 相关文献

[1]Characterization of a novel cry8 gene specific to Melolonthidae pests: Holotrichia oblita and Holotrichia parallela. Yan, Guixin,Zhang, Jie,Song, Fuping,Wang, Rongyan,Feng, Shuliang,Huang, Dafang.

[2]Genomic sequencing identifies novel Bacillus thuringiensis Vip1/Vip2 binary and Cry8 toxins that have high toxicity to Scarabaeoidea larvae. Zhang, Yanrui,Shu, Changlong,Song, Fuping,Zhang, Jie,Crickmore, Neil,Wang, Qinglei,Du, Lixin.

[3]Reduction of Bacillus thuringiensis Cry1Ac toxicity against Helicoverpa armigera by a soluble toxin-binding cadherin fragment. Liu, Chenxi,Wu, Kongming,Gao, Yulin,Ning, Changming,Wu, Yidong,Ning, Changming,Oppert, Brenda.

[4]Prediction of the key binding site of odorant-binding protein of Holotrichia oblita Faldermann ( Coleoptera: Scarabaeida). Zhuang, X.,Wang, Q.,Wang, B.,Zhong, T.,Cao, Y.,Li, K.,Yin, J.. 2014

[5]Identification and comparison of candidate odorant receptor genes in the olfactory and non-olfactory organs of Holotrichia oblita Faldermann by transcriptome analysis. Wei, Hongshuang,Shu, Changlong,Zhang, Shuai,Cao, Yazhong,Yin, Jiao,Luo, Chen. 2017

[6]Function and Immunocytochemical Localization of Two Novel Odorant-Binding Proteins in Olfactory Sensilla of the Scarab Beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae). Deng, Sisi,Yin, Jiao,Zhong, Tao,Cao, Yazhong,Li, Kebin. 2012

[7]A New Type I Peritrophic Membrane Protein from Larval Holotrichia oblita (Coleoptera: Melolonthidae) Binds to Chitin. Liu, Xiaomin,Guo, Wei,Liu, Xiaomin,Guo, Wei,Li, Ruijun,Zhao, Dan,Li, Xinna,Li, Jie. 2014

[8]Influence of Constant Temperature on Reproductive Parameters of Holotrichia oblita (Coleoptera: Scarabaeidae). Chen, Haoliang,Lin, Lulu,Xie, Minghui,Zhang, Guangling,Su, Weihua. 2015

[9]Calreticulin: conserved protein and diverse functions in plants. Jia, Xiao-Yun,He, Li-Heng,Li, Run-Zhi,Jia, Xiao-Yun,Jing, Rui-Lian.

[10]Antagonistic HLH/bHLH Transcription Factors Mediate Brassinosteroid Regulation of Cell Elongation and Plant Development in Rice and Arabidopsis. Zhang, Li-Ying,Bai, Ming-Yi,Zhu, Jia-Ying,Wang, Hao,Wang, Wenfei,Zhao, Jun,Yang, Hongjuan,Xu, Yunyuan,Lin, Wen-Hui,Chong, Kang,Wang, Zhi-Yong,Zhang, Li-Ying,Zhu, Jia-Ying,Wang, Hao,Wang, Wenfei,Zhao, Jun,Bai, Ming-Yi,Sun, Yu,Wang, Zhi-Yong,Wu, Jinxia,Zhang, Zhiguo,Sun, Xuehui,Lu, Tiegang,Kim, Soo-Hwan,Fujioka, Shozo.

[11]Gene expression of heart- and adipocyte-fatty acid-binding protein and correlation with intramuscular fat in Chinese chickens. Li, W. J.,Li, H. B.,Chen, J. L.,Zhao, G. P.,Zheng, M. Q.,Wen, J..

[12]Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza sativa L.. Qin, Qiu-lin,Liu, Jin-ge,Zhang, Zhen,Peng, Ri-he,Xiong, Ai-sheng,Yao, Quan-hong,Chen, Jian-min.

[13]Molecular Characterization and Expression Pattern of Two General Odorant Binding Proteins from the Diamondback Moth, Plutella xylostella. Zhang, Zhi-Chun,Wang, Man-Qun,Zhang, Guoan,Lu, Yao-Bin.

[14]Fluorescence competition assay for the assessment of green leaf volatiles and trans-beta-farnesene bound to three odorant-binding proteins in the wheat aphid Sitobion avenae (Fabricius). Yin, Jiao,Deng, Sisi,Li, Kebin,Cao, Yazhong.

[15]Effect of pyramiding Bt and CpTI genes on resistance of cotton to Helicoverpa armigera (Lepidoptera: Noctuidae) under laboratory and field conditions. Jinjie Cui,Junyu Luo,Wopke Van Der Werf,Yan Ma,Jingyuan Xia.

[16]Effects of Bt transgenic cotton lines on the cotton bollworm parasitoid Microplitis mediator in the laboratory. Liu, XX,Zhang, QW,Zhao, JZ,Li, HC,Xu, BL,Ma, XM. 2005

[17]Identification of ABCC2 as a binding protein of Cry1Ac onbrush border membrane vesicles from Helicoverpa armigera by an improved pull-down assay. Wang, Zeyu,Zhou, Xueping,Zhou, Zishan,Liu, Yuxiao,Liang, Gemei,Shu, Changlong,Song, Fuping,Zhou, Xueping,Zhang, Jie,Bravo, Alejandra,Soberon, Mario. 2016

[18]Processing of delta-endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 in Heliothis armigera midgut juice and the effects of protease inhibitors. Shao, ZZ,Cui, YL,Liu, XL,Yi, HQ,Ji, JH,Yu, ZN. 1998

[19]Recovery of Bacillus thuringiensis based biopesticides from fermented sludge by cross-flow microfiltration. Chang, Ming,Zhou, Shungui,Ni, Jinren,Chang, Ming,Sun, Qihong,Zhou, Shungui,Li, Tiexiang. 2012

[20]Diamondback moth resistance to insecticides in Guangdong Province. Feng, X,Chen, HY,Lu, LH. 2004

作者其他论文 更多>>

微信小程序