Identification and virulence characterization of entomopathogenic fungus Lecanicillium attenuatum against the pea aphid Acyrthosiphon pisum (Hemiptera: Aphididae)

文献类型： 外文期刊

第一作者： Wang, Dengke

作者： Wang, Dengke;Deng, Jianxin;Jin, Zhenyu;Wang, Wenkai;Dong, Xiaolin;Wang, Dengke;Pei, Yangfang;Li, Tian;Jin, Zhenyu;Liang, Ling;Wang, Wenkai;Dong, Xiaolin;Li, Liangde

作者机构：

关键词： Lecanicillium attenuatum;Acyrthosiphon pisum;ITS;Crude extract;Virulence

期刊名称：APPLIED ENTOMOLOGY AND ZOOLOGY （ 影响因子：1.403； 五年影响因子：1.381 ）

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收录情况： SCI

摘要： An entomopathogenic fungal strain was originally isolated on artificial medium from the corpse of a pea aphid (Acyrthosiphon pisum Harris) collected at Jingzhou, China (N30 degrees 21'18.15"Euro(3), E112 degrees 08'41.63"Euro(3)). Based on tests of the morphological, physiological and biochemical characteristics and analysis of internal transcribed spacer (ITS) sequences, it was considered to be a strain of Lecanicillium attenuatum Zare & W. Gams. Therefore, the strain was designated L. attenuatum YZU 151121. The activity of the biological agents under study was determined at 26 degrees C and 90% relative humidity. The number of A. pisum killed was increased by increasing the concentration of L. attenuatum. The results demonstrated that L. attenuatum YZU 151121 showed a high efficacy against 3rd-instar nymphs (LC50 = 2.91 +/- 0.365 x 10(5) conidia/ml) and adults (LC50 = 3.12 +/- 0.398 x 10(6) conidia/ml) after 6 days of exposure. Crude extract from this strain was tested for contact toxicity and showed high activity in 3rd-instar nymphs and adults, with LC50 values of 251.34 +/- 49.54 and 315.46 +/- 87.66 mg/l, respectively. In addition, crude extract at a concentration of 200 mg/l could significantly reduce fecundity in adults. These results revealed that the strain YZU 151121 may be useful in biopesticides for controlling pea aphid.

分类号： Q96

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