Comparison of Six Methylation Methods for Fatty Acid Determination in Yak Bone Using Gas Chromatography

文献类型： 外文期刊

第一作者： Jia, Wei

作者： Jia, Wei;Wu, Ting;Yu, Qunli;Jia, Wei;Liu, Wenyuan;Mi, Si;Zhang, Chunhui;Li, Xia;Wu, Ting;Yu, Qunli;Liu, Wenyuan;Yu, Qunli

作者机构：

关键词： Fatty acids;Yak bone;Methylation;Determination;Validation

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Yak bone is rich in nutrition but not used efficiently. Six methylation methods (M1: acetyl chloride-methanol method, M2: H2SO4-methanol method, M3: HCl-methanol method, M4: KOH-methanol method, M5: M2 in combination with M4, and M6: M3 in combination with M4) with (system 1 (S1)) or without (system 2 (S2)) prior acid hydrolysis for fatty acid (FA) determination using gas chromatography in yak bone were comprehensively compared. The contents of total FA determined by using M1 and M2 were significantly higher (p < 0.05) than those by using M3 for both S1 and S2 (119.83 versus 118.34 versus 38.31 mg/g in S1; 104.03 versus 93.73 versus 30.08 mg/g in S2 for M1 versus M2 versus M3, respectively). The result determined by M4, M5, and M6 was less than 5 mg/g. Validation results of M1 and M2 indicate that for most (> 90%) of the FA, the intra-day and inter-day CVs were < 9%. Recoveries for more than 90% of the FA were within a range of 85-117%. In addition, C18:1n9c, C16:0, C18:0, and conjugated linoleic acid were the major FAs in yak bone, approximately accounted for 50, 20, 10, and 10% of the total FA, respectively. And the ratio of saturated and unsaturated FA in yak bone was about 1:2. The acid hydrolysis/H2SO4-methanol esterification method would be more efficient for the accurate determination of FA in yak bone, whereas the direct acetyl chloride-methanol method would be used as an alternative for the fast analysis.

分类号： TS2

  • 相关文献

[1]A simple and economic method for simultaneous determination of 11 antibiotics in manure by solid-phase extraction and high-performance liquid chromatography. Feng, Yao,Zhang, Wenjuan,Liu, Yuanwang,Li, Zhaojun,Hu, Haiyan,Wei, Chaojun,Zhang, Wenjuan,Xue, Jianming. 2016

[2]Simple and cost-effective determination of ciprofloxacin hydrochloride by electrical micro-titration. Zhang, Xu-Zhi,Huang, Meng-Shi,Yang, Qian-Qian,Ding, Dong-Sheng,Zhao, Jun,Qu, Ke-Ming,Huang, Meng-Shi,Yang, Qian-Qian,Yang, Wen-Rong. 2017

[3]Advance in the Stripping Voltammetry Using Alloy Electrodes for the Determination of Heavy Metal Ions. Zhang, Xuzhi,Qu, Keming,Li, Dongpeng. 2015

[4]Rapid and Sensitive Determination of Dinotefuran Residue Based on Electrochemical Enhancement of beta-cyclodextrin-Graphene Composite. Zhang, M.,Zhai, X. C.,Yang, X.,Zhao, H. T.,Dong, A. J.,Zhang, H.,Wang, J.,Liu, G. Y.,Zhang, M.,Wang, J..

[5]Resonance light-scattering enhancement effect of the protein-Y3+-TTA-SLS system and its analytical application. Guo, Changying,Wu, Xia,Xu, Wei,Yang, Jinghe,Guo, Changying,Wu, Xia,Xu, Wei,Yang, Jinghe,Guo, Changying.

[6]Rapid and simple determination of polyphyllin I, II, VI, and VII in different harvest times of cultivated Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz by UPLC-MS/MS and FT-IR. Wu, Zhe,Zhang, Ji,Wang, Yuanzhong,Zhang, Jinyu,Wu, Zhe,Zhang, Ji,Wang, Yuanzhong,Zhang, Jinyu,Wu, Zhe,Xu, Furong,Zhang, Jinyu.

[7]Miniaturized liquid-liquid extraction coupled with ultra-performance liquid chromatography/tandem mass spectrometry for determination of topramezone in soil, corn, wheat, and water. Dong, Fengshou,Liu, Xingang,Xu, Jun,Li, Jing,Lu, Caihong,Zheng, Yongquan,Wang, Yunhao.

[8]Characterization of Tomato Transcription Factor WUSCHEL and Functional Study in Arabidopsis. Wang Xiang,Wang Xin-guo,Ren Jiang-ping,Ma Ying,Yin Jun,Wang Xiang. 2012

[9]Quantitative determination of soybean meal content in compound feeds: comparison of near-infrared spectroscopy and real-time PCR. Li, Hui,Lv, Xiaowen,Wang, Jing,Li, Junguo,Yang, Haifeng,Qin, Yuchang.

[10]Determination of Major and Trace Elements in Lotus Seed by Inductively Coupled Plasma-Mass Spectrometry. Zhang, Yongzhi,Wang, Gangjun,Ye, Xuezhu,Zheng, Chuangmu.

[11]Determination of Sudan Dyes Residues in the Brine by Liquid Chromatography. Guo, HongBin,Wang, MingYue,Lin, Ling,Yang, ChunLiang,Zhang, LiQiang,Li, Qi,Zeng, RiZhong. 2015

[12]Development of a multi-residue enantiomeric analysis method for 9 pesticides in soil and water by chiral liquid chromatography/tandem mass spectrometry. Dong, Fengshou,Liu, Xingang,Xu, Jun,Chen, Xiu,Han, Yongtao,Liang, Xuyang,Zheng, Yongquan.

[13]Highly sensitive and selective spectrofluorimetric determination of nitrite in food products with a novel fluorogenic probe. Huang, Haiwei,He, Lan,Wang, Qiuhua,Ma, Sufang,Li, Minfeng,Cao, Aocheng.

[14]A RAPID ELECTROCHEMICAL MONITORING PLATFORM FOR SENSITIVE DETERMINATION OF THIAMETHOXAM BASED ON beta-CYCLODEXTRIN-GRAPHENE COMPOSITE. Zhai, Xingchen,Yang, Xin,Wang, Jing,Zhai, Xingchen,Zhang, Hua,Zhang, Min,Yang, Xin,Gu, Cheng,Zhao, Haitian,Wang, Zhenyu,Dong, Aijun,Wang, Jing,Zhou, Guopeng. 2017

[15]Determination of sulfonamides in bovine milk by ultra performance liquid chromatography combined with quadrupole mass spectrometry. She, Yong-Xin,Liu, Jiajia,Wang, Jing,Wang, Rongyan,She, Yong-Xin,Liu, Yong,Cao, Weiqiang.

[16]Ultra-performance convergence chromatography (UPC2) method for the analysis of biogenic amines in fermented foods. Gong, Xiao,Qi, Ningli,Lin, Lijing,Li, Jihua,Wang, Xiaoxi. 2014

[17]Analysis of trace microcystins in vegetables using matrix solid-phase dispersion followed by high performance liquid chromatography triple quadrupole mass spectrometry detection. Qian, Zong-Yao,Li, Zhi-Gang,Ma, Jian,Gong, Ting-Ting,Xian, Qi-Ming,Qian, Zong-Yao.

[18]High Performance Liquid Chromatography determination of Fentin Acetate Residue in Beet and Soil. Sun Hui-qing,Li Yi-qiang,Xu Guang-jun,Zheng Xiao,Xu Jin-li,Wang Xiu-guo. 2012

[19]Determination of Iridoids in Gentiana rigescens by Infrared Spectroscopy and Multivariate Analysis. Qi, Lu-Ming,Qi, Lu-Ming,Zhang, Ji,Zuo, Zhi-Tian,Zhao, Yan-Li,Wang, Yuan-Zhong,Hang, Jin,Qi, Lu-Ming,Zhang, Ji,Zuo, Zhi-Tian,Zhao, Yan-Li,Wang, Yuan-Zhong,Hang, Jin.

[20]The effects of dietary betaine supplementation on fatty liver performance, serum parameters, histological changes, methylation status and the mRNA expression level of Spot14 alpha in Landes goose fatty liver. Su, S. Y.,Li, Q. F.,Wang, H. W.,Xie, Z.,Su, S. Y.,Wang, H. W.,Li, X. B.,Li, Q. F.,Dodson, M. V.. 2009

作者其他论文 更多>>

微信小程序