The Magnaporthe oryzae Alt A 1-like protein MoHrip1 binds to the plant plasma membrane

文献类型： 外文期刊

第一作者： Liang, Yingbo

作者： Liang, Yingbo;Dong, Yijie;Gao, Yuhan;Yang, Xiufen;Yuan, Jingjing;Qiu, Dewen

作者机构：

关键词： Alt A 1;Magnaporthe oryzae;MoHrip1;Microbe-associated molecular pattern (MAMP)

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： MoHrip1, a protein isolated from Magnaporthe oryzae, belongs to the Alt A 1 (AM) family. mohrip1 mRNA levels showed inducible expression throughout the infection process in rice. To determine the location of MoHrip1 in M. oryzae, a mohrip1-gfp mutant was generated. Fluorescence microscopy observations and western blotting analysis showed that MoHrip1 was both present in the secretome and abundant in the fungal cell wall. To obtain MoHrip1 protein, we carried out high-yield expression of MoHrip1 in Pichia pastoris. Treatment of tobacco plants with MoHrip1 induced the formation of necrosis, accumulation of reactive oxygen species and expression of several defense-related genes, as well as conferred disease resistance. By fusion to green fluorescent protein, we showed that MoHrip1 was able to bind to the tobacco and rice plant plasma membrane, causing rapid morphological changes at the cellular level, such as cell shrinkage and chloroplast disorganization. These findings indicate that MoHrip1 is a microbe-associated molecular pattern that is perceived by the plant immune system. This is the first study on an AA1 family protein that can bind to the plant plasma membrane. (C) 2017 Elsevier Inc. All rights reserved.

分类号： Q5

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