Isolation of two new retrotransposon sequences and development of molecular and cytological markers for Dasypyrum villosum (L.)

文献类型： 外文期刊

第一作者： Jiang, Yun

作者： Jiang, Yun;Guo, Yuanlin;Deng, Guangbing;Yu, Maoqun;Long, Hai;Xuan, Pu;Zhang, Jie

作者机构：

关键词： Dasypyrum villosum;Retrotransposon;SCAR marker;Karyotype;FISH

期刊名称：GENETICA （ 影响因子：1.082； 五年影响因子：1.489 ）

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年卷期：

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收录情况： SCI

摘要： Dasypyrum villosum is a valuable genetic resource for wheat improvement. With the aim to efficiently monitor the D. villosum chromatin introduced into common wheat, two novel retrotransposon sequences were isolated by RAPD, and were successfully converted to D. villosum-specific SCAR markers. In addition, we constructed a chromosomal karyotype of D. villosum. Our results revealed that different accessions of D. villosum showed slightly different signal patterns, indicating that distribution of repeats did not diverge significantly among D. villosum accessions. The two SCAR markers and FISH karyotype of D. villosum could be used for efficient and precise identification of D. villosum chromatin in wheat breeding.

分类号： Q3

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