Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis

文献类型： 外文期刊

第一作者： Chen, Chen

作者： Chen, Chen;Li, Chenlong;Wang, Ying;Renaud, Justin;Tian, Gang;Kambhampati, Shrikaar;Saatian, Behnaz;Vi Nguyen;Hannoufa, Abdelali;Marsolais, Frederic;Yuan, Ze-Chun;Austin, Ryan S.;Liu, Jun;Cui, Yuhai;Chen, Chen;Kambhampati, Shrikaar;Saatian, Behnaz;Hannoufa, Abdelali;Marsolais, Frederic;Austin, Ryan S.;Kohalmi, Susanne E.;Cui, Yuhai;Li, Chenlong;Huang, Shangzhi;Li, Chenlong;Huang, Shangzhi;Yu, Kangfu;Liu, Jun;Wu, Keqiang

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期刊名称：NATURE PLANTS （ 影响因子：15.793； 五年影响因子：17.349 ）

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收录情况： SCI

摘要： Acetyl-coenzyme A (acetyl-CoA) is a central metabolite and the acetyl source for protein acetylation, particularly histone acetylation that promotes gene expression. However, the effect of acetyl-CoA levels on histone acetylation status in plants remains unknown. Here, we show that malfunctioned cytosolic acetyl-CoA carboxylase1 (ACC1) in Arabidopsis leads to elevated levels of acetyl-CoA and promotes histone hyperacetylation predominantly at lysine 27 of histone H3 (H3K27). The increase of H3K27 acetylation (H3K27ac) is dependent on adenosine triphosphate (ATP)-citrate lyase which cleaves citrate to acetyl-CoA in the cytoplasm, and requires histone acetyltransferase GCN5. A comprehensive analysis of the transcriptome and metabolome in combination with the genome-wide H3K27ac profiles of acc1 mutants demonstrate the dynamic changes in H3K27ac, gene transcripts and metabolites occurring in the cell by the increased levels of acetyl-CoA. This study suggests that H3K27ac is an important link between cytosolic acetyl-CoA level and gene expression in response to the dynamic metabolic environments in plants.

分类号： Q94

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