Development of SSR molecular markers for Allium mongolicum

文献类型： 外文期刊

第一作者： Hu, Jing

作者： Hu, Jing;Hu, Xiaoke;Zhang, Jinhu;Fan, Baoli;Yu, Qiushi;Zhang, Qian

作者机构：

关键词： Allium mongolicum;Genomic DNA;SSR molecular markers;Genetic diversity;Polymorphism

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

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收录情况： SCI

摘要： Allium mongolicum is high palatability, nutrient, medicinal value and drought resistance wild plant. However, a lack of molecular markers of this plant results in a series of genetic questions remain largely unknown, including molecular marker data, population genetic structure and accuracy of genetic breeding. In this study, a total of 1,409,706 quality-filtered and trimmed reads were obtained from the normalized genomic DNA of pooled A. mongolicum individuals. These sequences were assembled into 2,093,593 high quality contigs, and a total of 219 simple sequence repeats loci were screened. Sixty of them were selected to validate amplification and to determine the degree of polymorphism in the genomic DNA pools. Fifteen primer pairs successfully amplified DNA fragments and detected significant amounts of polymorphism. The number of alleles in ten geographically diverse A. mongolicum populations ranged from 3 to 8 per locus. The observed and expected heterozygosities ranged from 0.350 to 0.860 and 0.516 to 0.931, respectively. Our results provide a valuable resource for A. mongolicum research.

分类号： Q

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