A cell clone strain from Mythimna separata (Lepidoptera: Noctuidae) highly susceptible to Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and M. separata NPV (MsNPV)

文献类型： 外文期刊

第一作者： Zheng, Gui-Ling

作者： Zheng, Gui-Ling;Zhao, Chuan-De;Wan, Fang-Hao;Li, Chang-You;Meng, Xiang-Qian;Wan, Fang-Hao

作者机构：

关键词： Mythimna separata;Cell clone strain;Autographa californica multiple nucleopolyhedrovirus (AcMNPV);Mythimna separata nucleopolyhedrovirus (MsNPV)

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, we describe a cell line, Ms-10C, cloned from the line QAU-Ms-E-10 (simplified Ms-10), an embryonic line from Mythimna separata. The cloned cell line was significantly more sensitive to nucleopolyhedrovirus (NPV). Ms-10C cells were mainly spherical with a diameter of 14.42 +/- 2.23 mu m. DNA amplification fingerprinting (DAF) confirmed the profile of PCR-amplified bands of the cloned cell line was consistent with those of the parental cell line, Ms-10. The sequencing result of the mitochondrial cytochrome c oxidase I (mtCO I) fragment confirmed that the amplified 636-bps mtCOI fragment was 100% identical to that of M. separata. Its chromosomes exhibited the typical characters of lepidopteran cell lines. Its population doubling time was 42.2 h at 27 degrees C. Ms-10C was more sensitive than Ms-10 to both Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and M. separata nucleopolyhedrovirus (MsNPV). At 4 d post infection, the infection rates of two viruses reached 94.2 and 92.3%, respectively. The availability of this cell clone strain will provide a useful tool for the basic research on nucleopolyhedrovirus and for potential application in expression of recombinant proteins with baculovirus expression vector system.

分类号： Q95

  • 相关文献

[1]Host age preference of Microplitis mediator (Hymenoptera : Braconidae), an endoparasitoid of Mythimna separata (Lepidoptera : Noctuidae). Li, Jiancheng,Coudron, Thomas A.,Pan, Wenliang,Liu, Xiaoxia,Lu, Ziyun,Zhang, Qingwen. 2006

[2]The role of nectar plants in severe outbreaks of armyworm Mythimna separata (Lepidoptera : Noctuidae) in China. Zhang, Qing-Wen,Ye, Zhi-Hua,Luo, Li-Zhi. 2006

[3]Autumn migration of Mythimna separata (Lepidoptera : Noctuidae) over the Bohai Sea in northern China. Zhao, Xin-Cheng,Wu, Xian-Fu,Wu, Bo,Wu, Kong-Ming,Cheng, Deng-Fa,Guo, Yu-Yuan,Feng, Hong-Qiang. 2008

[4]Identification and Functional Analysis of Chitin Synthase A in Oriental Armyworm, Mythimna separata. Zhai, Yifan,Yin, Zhenjuan,Men, Xingyuan,Zheng, Li,Zhai, Yifan,Fan, Xiaobin,Yue, Xiangzhao,Zhang, Wenqing,Zhai, Yifan,Fan, Xiaobin,Yue, Xiangzhao,Zhang, Wenqing. 2017

[5]Does the onset of sexual maturation terminate the expression of migratory behaviour in moths? A study of the oriental armyworm, Mythimna separata. Zhao, Xin Cheng,Wu, Bo,Wu, Xian Fu,Liu, Zhong Fang,Wu, Kong Ming,Feng, Hong Qiang,McNeil, Jeremy N..

[6]Central Projections of Antennal and Labial Palp Sensory Neurons in the Migratory Armyworm Mythimna separata. Zhao, Xin-Cheng,Tang, Qing-Bo,Berg, Bente G.,Xie, Gui-Ying,Wang, Gui-Rong. 2017

[7]Seasonal cues mediate host behaviour modification and cocoon polymorphism in Microplitis mediator (Hymenoptera: Braconidae). Zhang, Jing,Liu, Xiao-Xia,Zhang, Qing-Wen,Michaud, J. P.,Li, Jian-Cheng,Luo, Shu-Ping.

[8]Sterol content in the artificial diet of Mythimna separata affects the metabolomics of Arma chinensis (Fallou) as determined by proton nuclear magnetic resonance spectroscopy. Liu, Chen-xi,Zhang, Li-sheng,Wang, Meng-qing,Chen, Hong-yin,Guo, Yi,Liu, Chen-xi,Zhang, Li-sheng,Wang, Meng-qing,Chen, Hong-yin.

[9]Effect of leaf age on flight response of a parasitic wasp Cotesia kariyai (Hymenoptera : Braconidae) to a plant-herbivore complex. Hou, M,Takabayashi, J,Kainoh, Y. 2005

[10]Relative fitness of near isogenic lines for melanie and typical forms of the oriental armyworm, Mythimna separata (Walker). Luo, Li-Zhi,Zhang, Lei. 2007

[11]Complete mitochondrial genome of the oriental armyworm Mythimna separata (Walker) (Lepidoptera: Noctuidae). Li, Feng-Bo,Wang, Wei,Zhang, Hui-Xian,Shen, Wei-Feng,Chen, Jin-E,Meng, Zhi-Qi,Wang, Wei,Zhang, Hui-Xian,Xu, Xiao-Yan. 2015

[12]Regulation of Migration in Mythimna separata (Walker) in China: A Review Integrating Environmental, Physiological, Hormonal, Genetic, and Molecular Factors. Jiang, Xingfu,Luo, Lizhi,Zhang, Lei,Hu, Yi,Sappington, Thomas W.. 2011

[13]De novo analysis of the oriental armyworm Mythimna separata antennal transcriptome and expression patterns of odorant-binding proteins. Chang, Xiang-Qian,Nie, Xiao-Pei,Zhang, Zan,Zeng, Fang-Fang,Wang, Man-Qun,Chang, Xiang-Qian,Lv, Liang,Zhang, Shu.

[14]Amplified fragment length polymorphism analysis of Mythimna separata (Lepidoptera : Noctuidae) geographic and melanie laboratory populations in China. Luo, Li-Zhi,Zhang, Lei.

作者其他论文 更多>>

微信小程序