Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile
文献类型: 外文期刊
第一作者: Fan, Haiyang
作者: Fan, Haiyang;Chen, Lifeng;Wang, Hualei;Liu, Qinghai;Ren, Yuhong;Wei, Dongzhi;Sun, Huihui
作者机构:
关键词: Biotransformation;Fed-batch reaction;Fermentation;Nitrilase;Phenylacetic acid
期刊名称:CHEMICAL PAPERS ( 影响因子:2.097; 五年影响因子:1.831 )
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收录情况: SCI
摘要: This study aimed at developing an efficient biotransformation process for phenylacetic acid production from phenylacetonitrile by using recombinant Escherichia coli M15 harboring a double mutant MG nitrilase (I113M/Y199G) from Burkholderia cenocepacia J2315. A yield of 2310 U/mL nitrilase was obtained by fermentation after the optimization of cultivation conditions, with a specific activity of 64 U/mg dcw. The MG nitrilase showed high substrate tolerance and completely hydrolyzed 100 mM phenylacetonitrile in 30 min under optimal conditions. To alleviate substrate inhibition, periodic or continuous batch-feeding of substrate was used during the biotransformation. Up to 164 g/L substrate was completely hydrolyzed in 9 h with continuous batch-feeding using resting cells, corresponding to 400 U/mL of nitrilase activity, and leading to production of 163.4 g/L phenylacetic acid. The hydrolysis process has potential application for phenylacetic acid production on a large scale.
分类号: O6
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