Effect of porcine glucagon-like peptides-2 on tight junction in GLP-2R+IPEC-J2 cell through the PI(3)k/Akt/mTOR/p70(S6K) signalling pathway

文献类型: 外文期刊

第一作者: Qi, K. K.

作者: Qi, K. K.;Sun, Y. Q.;Wan, J.;Deng, B.;Men, X. M.;Wu, J.;Xu, Z. W.

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关键词: porcine glucagon-like peptide-2;IPEC-J2;tight junction protein;PI(3)k;Akt;mTOR;p70(S6K)

期刊名称:JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION ( 影响因子:2.13; 五年影响因子:2.322 )

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收录情况: SCI

摘要: Because of rare glucagon-like peptide-2 (GLP-2) receptor (+) cells within the gut mucosa, the molecular mechanisms transducing the diverse actions of GLP-2 remain largely obscure. This research identified the naturally occurring intestinal cell lines that endogenously express GLP-2R and determined the molecular mechanisms of the protective effects of GLP-2-mediated tight junctions (TJ) in GLP-2R (+) cell line. (i) Immunohistochemistry results showed that GLP-2R is localised to the epithelia, laminae propriae and muscle layers of the small and large bowels of newborn piglets. (ii) GLP-2R expression was apparent in the cytoplasm of endocrine cells in IPEC-J2 cell lines. (iii) The protein expressions of ZO-1, claudin-1, occludin, p-PI3K, p-Akt, p-mTOR and p-p70(S6K) significantly (p<0.05) increased in GLP-2-treated IPEC-J2 cells, and all of them significantly (p<0.05) decreased when LY-294002 or rapamycin was added. GLP-2 improves intestinal TJ expression of GLP-2R (+) cells through the PI(3)k/Akt/mTOR/p70(S6K) signalling pathway.

分类号: S8

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