Hybrid Purity Testing of Brassica rapa Using SSR Marker Technology

文献类型： 外文期刊

第一作者： Li, Li

作者： Li, Li;Liu, Ling;Wu, Ping;Xu, Xiulan;Li, Li;Liu, Ling;Zhang, Deshuang;Wu, Ping;Zhang, Fenglan;Xu, Xiulan;Li, Li;Liu, Ling;Zhang, Deshuang;Wu, Ping;Zhang, Fenglan;Xu, Xiulan;Li, Li;Liu, Ling;Zhang, Deshuang;Wu, Ping;Zhang, Fenglan;Xu, Xiulan;Li, Li;Liu, Ling;Zhang, Deshuang;Wu, Ping;Zhang, Fenglan;Xu, Xiulan

作者机构：

关键词： Brassica rapa ssp pekinensis;Brassica rapa chinensis group;hybrid;SSR markers;purity identification;special strain

期刊名称：HORTSCIENCE （ 影响因子：1.455； 五年影响因子：1.617 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Thirteen Chinese cabbage (Brassica rapa) hybrid cultivars and 26 parental inbred lines were used as experimental materials to screen for primers producing hybrid and parental complementary bands and for primers with high polymorphism information contents and low genotype frequencies. A total of 18 pairs of core primers were designed to identify the purity of Chinese cabbage. There was no significant difference in the purity percentage measured between different loci of the same strain. The fingerprint obtained by the amplification of each locus could be used to identify purity to obtain an authentic purity percentage. Curve mapping and significance analyses were conducted using the purity percentage of eight different seed samples and confirmed a sampling seed number of 96. The results of the purity test were verified by comparison with the grow-out test (GOT) using molecular markers. In conclusion, the simple sequence repeat (SSR) detection system could be used for the rapid identification of the purity of the tested Chinese cabbage hybrids.

分类号： S6

  • 相关文献

[1]Bee Products Quality Control and Emergency Management Mechanism Research Based on Multi-Agent. Yue, E.,Zhu, Yeping,Cao, Yongsheng. 2012

[2]The miRNAs and their regulatory networks responsible for pollen abortion in Ogura-CMS Chinese cabbage revealed by high-throughput sequencing of miRNAs, degradomes, and transcriptomes. Wei, Xiaochun,Yao, Qiuju,Yuan, Yuxiang,Zhao, Yanyan,Zhang, Qiang,Wang, Zhiyong,Jiang, Wusheng,Zhang, Xiaowei,Zhang, Xiaohui,Li, Xixiang,Wei, Fang. 2015

[3]Genome-wide identification and characterization of aquaporin genes (AQPs) in Chinese cabbage (Brassica rapa ssp pekinensis). Tao, Peng,Zhong, Xinmin,Li, Biyuan,Wang, Wuhong,Yue, Zhichen,Lei, Juanli,Huang, Xiaoyun,Guo, Weiling,Huang, Xiaoyun.

[4]Construction of a sequence-based bin map and mapping of QTLs for downy mildew resistance at four developmental stages in Chinese cabbage (Brassica rapa L. ssp pekinensis). Yu, Shuancang,Su, Tongbing,Zhi, Shenghua,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Zhao, Xiuyun,Yu, Yangjun,Yu, Shuancang,Su, Tongbing,Zhi, Shenghua,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Zhao, Xiuyun,Yu, Yangjun,Yu, Shuancang,Su, Tongbing,Zhi, Shenghua,Zhang, Fenglan,Wang, Weihong,Zhang, Deshuang,Zhao, Xiuyun,Yu, Yangjun.

[5]Body color development and genetic analysis of hybrid transparent crucian carp (Carassius auratus). Xu, W.,Tong, G. X.,Geng, L. W.,Jiang, H. F.. 2015

[6]Development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid VP2 protein carrying immunoreactive epitope of porcine circovirus type 2. Pan, Qunxing,He, Kongwang,Pan, Qunxing,Huang, Kehe. 2008

[7]Genetic contribution of Chinese landraces to the development of sorghum hybrids. Li, Y,Li, CZ. 1998

[8]Mitochondrial DNA sequence of the hybrid of Leiocassis longirostris (female) and Pelteobagrus fulvidraco (male). Guo, Shan-shan,Luo, Xiang-zhong,Liang, Hong-wei,Liang, Hong-wei. 2016

[9]Dissection of combining ability for yield and related traits using introgression lines in the background of a key restorer line in rice (Oryza sativa L.). Xiang, Chao,Zhang, Hongjun,Wang, Jie,Wang, Wensheng,Gao, Yongming,Wang, Hui,Xia, Jiafa,Ye, Guoyou.

[10]Karyotype Analysis of Mediterranean Buffalo and Its Hybrids. Huang, Fenxiang,Yang, Bingzhuang,Tan, ZhengZhun,Huang, Jian,Li, Hui,Pang, Chunying,Shang, Jianghua,Liang, Xianwei. 2013

[11]Breeding of new pear cultivar 'Huasu'. Fang, CQ,Chen, XY,Lin, SH. 2002

[12]Rapid Identification of Peanut Hybrids with SSR-PCR. Hong Yanbin,Li Xingyu,Huang Shangzhi,Hong Yanbin,Chen Xiaoping,Liang Xuanqiang,Liu Hong. 2015

[13]Different Aluminum Tolerance among Indica, Japonica and Hybrid Rice Varieties. Shu Chang,Wu Jing-hao,Shi Gao-ling,Lou Lai-qing,Deng Jun-xia,Cai Qing-sheng,Wan Jian-lin. 2015

[14]A comparative study of two-line early season hybrid rice with lepidopteran resistance. Yi, Zili,Meng, Qiucheng,Weng, Lushui,Yu, Jianghui,Xiao, Guoying,Liu, Wenqiang,Peng, Jian,Xiao, Youlun.

[15]Advances in Sino-Thai Cooperation in Peach Breeding. Zhao, J. B.,Guo, J. Y.,Ren, F.,Wang, Z.,Jiang, Q.,Sripinta, P.,Daoruang, C.,Boonprakob, U.,Nopkoonwong, U..

[16]QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli. Asad Muhammad Azeem,BAI Bin,LAN Cai-xia,YAN Jun,XIA Xian-chun,ZHANG Yong,HE Zhong-hu. 2013

[17]Identification of stable quantitative trait loci (QTLs) for fiber quality traits across multiple environments in Gossypium hirsutum recombinant inbred line population. Muhammad Jamshed,Fei Jia,Juwu Gong,;Koffi Kibalou Palanga,Yuzhen Shi,Junwen Li,Haihong Shang,Aiying Liu,Tingting Chen,Zhen Zhang,Juan Cai,Qun Ge,Zhi Liu,Quanwei Lu,Xiaoying Deng,Yunna Tan,Harun or Rashid,Zareen Sarfraz,Murtaza Hassan,Wankui Gong,Youlu Yuan. 2016

[18]Availability of Two-dimensional Bulked DNA Sampling in Uniformity Assessment of 8 Inbred Rice Lines. Liu Yan-Fang,Huang Qing-Mei,Yang Xiao-Hong,Li Yan-Gang,Zhang Peng,Wang Jiang -Min,Zhang Hui,Guan Jun-Jiao,Zhang Jian-Hua. 2016

[19]Fingerprinting 128 Chinese clonal tea cultivars using SSR markers provides new insights into their pedigree relationships. Tan, Li-Qiang,Peng, Min,Xu, Li-Yi,Chen, Sheng-Xiang,Zou, Yao,Qi, Gui-Nian,Wang, Li-Yuan,Cheng, Hao,Wang, Li-Yuan. 2015

[20]Chromosomal location of powdery mildew resistance gene Pm16 in wheat using SSR marker analysis. Chen, XM,Luo, YH,Xia, XC,Xia, LQ,Chen, X,Ren, ZL,He, ZH,Jia, JZ. 2005

作者其他论文 更多>>

微信小程序