Isolation, culture and biological characteristics of multipotent porcine skeletal muscle satellite cells

文献类型： 外文期刊

第一作者： Yang, Jinjuan

作者： Yang, Jinjuan;Liu, Hao;Wang, Kunfu;Li, Lu;Yuan, Hongyi;Liu, Xueting;Guan, Weijun;Yang, Jinjuan;Liu, Yingjie

作者机构：

关键词： Satellite cells;Self-renewal;Multidifferentiation potential;Biological characteristics;Porcine

期刊名称：CELL AND TISSUE BANKING （ 影响因子：1.522； 五年影响因子：1.968 ）

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年卷期：

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收录情况： SCI

摘要： Skeletal muscle has a huge regenerative potential for postnatal muscle growth and repair, which mainly depends on a kind of muscle progenitor cell population, called satellite cell. Nowadays, the majority of satellite cells were obtained from human, mouse, rat and other animals but rarely from pig. In this article, the porcine skeletal muscle satellite cells were isolated and cultured in vitro. The expression of surface markers of satellite cells was detected by immunofluorescence and RT-PCR assays. The differentiation capacity was assessed by inducing satellite cells into adipocytes, myoblasts and osteoblasts. The results showed that satellite cells isolated from porcine tibialis anterior were subcultured up to 12 passages and were positive for Pax7, Myod, c-Met, desmin, PCNA and NANOG but were negative for Myogenin. Satellite cells were also induced to differentiate into adipocytes, osteoblasts and myoblasts, respectively. These findings indicated that porcine satellite cells possess similar biological characteristics of stem cells, which may provide theoretical basis and experimental evidence for potential therapeutic application in the treatment of dystrophic muscle and other muscle injuries.

分类号： Q2

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