Isolation and genetic characterization of a novel adeno-associated virus from Muscovy ducks in China

文献类型: 外文期刊

第一作者: Su, X. N.

作者: Su, X. N.;Zhang, X. H.;Xie, Q. M.;Chen, W. G.;Chen, F.;Su, X. N.;Zhang, X. H.;Xie, Q. M.;Chen, W. G.;Chen, F.;Su, X. N.;Zhang, X. H.;Xie, Q. M.;Chen, F.;Su, X. N.;Zhang, X. H.;Xie, Q. M.;Chen, W. G.;Chen, F.;Su, X. N.;Zhang, X. H.;Xie, Q. M.;Chen, F.;Liu, J. J.;Zhou, Q. F.;Zhao, L. C.

作者机构:

关键词: novel;duck adeno-associated virus;sequence analysis;phylogenetic analysis

期刊名称:POULTRY SCIENCE ( 影响因子:3.352; 五年影响因子:3.679 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Adeno-associated virus (AAV; genus Dependoparvovirus, family Parvoviridae) was first discovered in 1965 as a contaminant in adenovirus preparations. The AAVs are generally considered nonpathogenic, and they have the ability to attenuate the replication of other more pathogenic viruses, which makes them attractive as potential therapeutics or preventative measures. This study characterized a novel AAV isolated from Muscovy ducks in China. The novel virus (MHH-05-2015) was isolated after propagating a field isolate of the DAdV-3 virus (a type 3 duck adenovirus) in duck embryo fibroblasts. The full genome sequence of MHH-05-2015 was determined, and the nucleotide and amino acid sequences were compared to other avian AAVs. The genomic distribution of the structural and non-structural protein-coding genes in MHH-05-2015 was conserved and consistent with the other AAVs. Compared to previously isolated avian AAVs, MHH-05-2015 had approximately 63 to 64% sequence identity. Phylogenetic analysis indicated that MHH-05-2015 clustered separately from other avian AAVs, suggesting that MHH-05-2015 was not directly descended from other Dependoparvovirus family members. These results suggest that MHH-05-2015 is a new subtype of AAV that is distinct from other avian AAVs.

分类号: S8

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