PxAPN5 serves as a functional receptor of Cry2Ab in Plutella xylostella (L.) and its binding domain analysis
文献类型: 外文期刊
第一作者: Pan, Zhi-Zhen
作者: Pan, Zhi-Zhen;Liu, Bo;Chen, Zheng;Zhu, Yu-Jing;Xu, Lian;Zhang, Jing;Chen, Qing-Xi
作者机构:
关键词: Bacillus thuringiensis;Cry2Ab;Plutella xylostella;AminopeptidaseN 5;Binding region
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Lepidopteran midgut aminopeptidases N (APNs) are widely studied for their potential roles as one of the receptors for Bacillus thuringiensis (Bt) crystal toxins. In the present study, a loss of function analyses by RNAi (RNA interference) silencing of the Plutella xylostella APN5 (PxAPN5), a binding protein of Bt crystal toxin Cry2Ab, were performed. The knocking down of PxAPN5 in P. xylostella larvae greatly reduced their susceptibility to Cry2Ab and led to a decrease of Cry2Ab binding to P. xylostella midgut. Four truncated fragments of PxAPN5 were further constructed and expressed in Escherichia coli (E.coli) to find the binding region of PxAPN5 to Cry2Ab. The ligand blot result indicated that D1 domain (residues 1-262) and D3 domain (residues 510-620) of PxAPN5 could specially bind to Cry2Ab. (C) 2017 Elsevier B.V. All rights reserved.
分类号: Q71
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