Expression and Purification of a Functional Porcine Soluble Triggering Receptor Expressed on Myeloid Cells 1

文献类型: 外文期刊

第一作者: Li, Hui

作者: Li, Hui;Yan, Leyan;Meng, Chunhua;Shi, Zhendan;Guo, Shuangshuang;Hu, Yiyi;He, Kongwang

作者机构:

关键词: Cytokines;Inflammatory responses;LPS;Porcine;TREM-1

期刊名称:ANIMAL BIOTECHNOLOGY ( 影响因子:2.282; 五年影响因子:1.702 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Triggering receptor expressed on myeloid cells 1 (TREM-1) plays a vital role in the pathogen-triggered amplification loop required for proinflammatory responses. Blockade of TREM-1 signaling may inhibit expansion of sepsis and prolong survival of animals. In the present study, the gene of porcine soluble TREM-1 was cloned and expressed in E. coli. After purification, the bioactivity of recombinant porcine soluble TREM-1 was tested in vitro on porcine alveolar macrophages. The results showed that supplementation with the recombinant porcine sTREM-1 protein rapidly and dose-dependently attenuated the upregulation of cytokines (IL-1, IL-2, IL-4, IL-8, IL-10, IL-12, IL-16, IL-18, and TNF-) caused by LPS stimulation in the cultured porcine alveolar macrophages. These results indicate that the recombinant porcine sTREM-1 protein can prevent TREM-1-mediated hyperinflammatory responses after exposure to LPS.

分类号: Q95

  • 相关文献

[1]Strong, sudden cooling alleviates the inflammatory responses in heat-stressed dairy cows based on iTRAQ proteomic analysis. Cheng, Jianbo,Fan, Caiyun,Cheng, Jianbo,Min, Li,Zheng, Nan,Zhao, Shengguo,Zhang, Yangdong,Wang, Jiaqi,Min, Li. 2018

[2]INFLAMMATION-RELATED OXIDATIVE STRESS IN WHITE ADIPOSE TISSUES OF AN INBRED OBESE PIG. Yang, X. F.,Jiang, Z. Y.,Tian, Z. M.,Qiu, Y. Q.,Wang, L.,Gao, K. G.,Hu, Y. J.,Ma, X. Y.. 2017

[3]Koumine Attenuates Lipopolysaccaride-Stimulated Inflammation in RAW264.7 Macrophages, Coincidentally Associated with Inhibition of NF-kappa B, ERK and p38 Pathways. Wu, Jing,Sun, Zhiliang,Yuan, Zhihang,Wu, Jing,Sun, Zhiliang,Matias, Froilan Bernard,Liang, Zengenni. 2016

[4]Dietary soy isoflavone attenuated growth performance and intestinal barrier functions in weaned piglets challenged with lipopolysaccharide. Zhu, Cui,Jiang, Zongyong,Zheng, Chuntian,Wang, Li,Yang, Xuefen,Ma, Xianyong,Gao, Kaiguo,Hu, Youjun,Zhu, Cui,Wu, Yunpeng. 2015

[5]Breed-linked polymorphisms of porcine toll-like receptor 2 (TLR2) and TLR4 and the primary investigation on their relationship with prevention against Mycoplasma pneumoniae and bacterial LPS challenge. Fang, Xiaomin,Liu, Xiao,Meng, Cui,Fu, Yanfeng,Wang, Xuemin,Li, Bixia,Tu, Feng,Zhao, Fang,Ren, Shouwen.

[6]Palmatine inhibits TRIF-dependent NF-kappa B pathway against inflammation induced by LPS in goat endometrial epithelial cells. Yan, Baoqi,Wang, Dongsheng,Dong, Shuwei,Sang, Mengqi,Yang, Zhiqiang,Zhang, Shidong,Yan, Zuoting,Na, Lidong,Yang, Hongzao,Cheng, Zhangrui.

[7]Aqueous extract of Bai-Hu-Tang, a classical Chinese herb formula, prevents excessive immune response and liver injury induced by LPS in rabbits. Zhang, Shidong,Wang, Dongsheng,Wang, Xurong,Yan, Zuoting,Zhang, Shidong,Wang, Dongsheng,Li, Shihong,Li, Jingyu,Zhang, Shidong,Wang, Xurong,Li, Jingyu,Li, Hongsheng,Yan, Zuoting,Zhang, Shidong,Li, Shihong,Li, Hongsheng. 2013

[8]Anti-Inflammatory Activity of Saponins from Quinoa ( Chenopodium quinoa Willd.) Seeds in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages Cells. Yao, Yang,Yang, Xiushi,Shi, Zhenxing,Ren, Guixing,Yang, Xiushi. 2014

[9]Six Alkaloids Inhibit Secretion of IL-1 alpha, TXB2, ET-1 and E-selectin in LPS-induced Endothelial Cells. Hu, Yi-Yi,He, Kong-Wang,Guo, Rong-li. 2012

[10]Chinese herbal medicinal ingredients affect secretion of NO, IL-10, ICAM-1 and IL-2 by endothelial cells. Hu, Yiyi,He, Kongwang,Zhu, Haodan,Hu, Yiyi,He, Kongwang,Zhu, Haodan.

[11]Anti-endotoxin and anti-inflammatory effects of Chinese herbal medicinal alkaloid ingredients in vivo. Hu, Yiyi,Mao, Aihua,Yu, Zhengyu,He, Kongwang,Hu, Yiyi,Mao, Aihua,Yu, Zhengyu,He, Kongwang.

[12]Protective effects of sinomenine against LPS-induced inflammation in piglets. Yang, Haifeng,Jiang, Chunmao,Chen, Xiaolan,He, Kongwang,Hu, Yiyi.

[13]Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes. Zhang, WY,Moreau, E,Yang, BZ,Li, ZQ,Hope, JC,Howard, CJ,Huang, WY,Chauvin, A. 2006

[14]Effects of different cytokines on immune responses of rainbow trout in a virus DNA vaccination model. Cao, Yongsheng,Xu, Liming,Li, Shaowu,Wang, Di,Zhao, Jingzhuang,Liu, Hongbai,Lu, Tongyan,Cao, Yongsheng,Zhang, Qiya,Feng, Jian. 2017

[15]Infection of Goose with Genotype VIId Newcastle Disease Virus of Goose Origin Elicits Strong Immune Responses at Early Stage. Xu, Qianqian,Chen, Yuqiu,Zhao, Wenjun,Zhang, Tingting,Liu, Chenggang,Qi, Tianming,Ma, Deying,Xu, Qianqian,Chen, Yuqiu,Zhao, Wenjun,Zhang, Tingting,Liu, Chenggang,Qi, Tianming,Han, Zongxi,Shao, Yuhao,Liu, Shengwang. 2016

[16]Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo. Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Dai, Manman,Shi, Meiqing. 2016

[17]Effects of Acanthopanax senticosus Polysaccharide Supplementation on Growth Performance, Immunity, Blood Parameters and Expression of Pro-inflammatory Cytokines Genes in Challenged Weaned Piglets. Han, Jie,Bian, Lianquan,Liu, Xianjun,Zhang, Fei,Zhang, Yiran,Yu, Ning. 2014

[18]Proteomic analysis of Fasciola hepatica excretory and secretory products (FhESPs) involved in interacting with host PBMCs and cytokines by shotgun LC-MS/MS. Liu, Qing,Huang, Si-Yang,Yue, Dong-Mei,Wang, Jin-Lei,Zhu, Xing-Quan,Liu, Qing,Wang, Yujian,Li, Xiangrui,Huang, Si-Yang,Zhu, Xing-Quan.

[19]Parapoxvirus orf virus infection induces an increase in interleukin-8, tumour necrosis factor-alpha, and decorin in goat skin fibroblast cells. Wang, Lingling,Lu, Bingzhou,Zheng, Haixue,Zhang, Keshan,Liu, Xiangtao.

[20]Characterisation and immunostimulatory activity of an alpha-(1 -> 6)-D-glucan from the cultured Armillariella tabescens mycelia. Yu, Mengyao,Yang, Zhirong,Zheng, Linyong,Xu, Xiaoyan,Yu, Mengyao,Zheng, Linyong. 2008

作者其他论文 更多>>