Expression and Purification of a Functional Porcine Soluble Triggering Receptor Expressed on Myeloid Cells 1
文献类型: 外文期刊
第一作者: Li, Hui
作者: Li, Hui;Yan, Leyan;Meng, Chunhua;Shi, Zhendan;Guo, Shuangshuang;Hu, Yiyi;He, Kongwang
作者机构:
关键词: Cytokines;Inflammatory responses;LPS;Porcine;TREM-1
期刊名称:ANIMAL BIOTECHNOLOGY ( 影响因子:2.282; 五年影响因子:1.702 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Triggering receptor expressed on myeloid cells 1 (TREM-1) plays a vital role in the pathogen-triggered amplification loop required for proinflammatory responses. Blockade of TREM-1 signaling may inhibit expansion of sepsis and prolong survival of animals. In the present study, the gene of porcine soluble TREM-1 was cloned and expressed in E. coli. After purification, the bioactivity of recombinant porcine soluble TREM-1 was tested in vitro on porcine alveolar macrophages. The results showed that supplementation with the recombinant porcine sTREM-1 protein rapidly and dose-dependently attenuated the upregulation of cytokines (IL-1, IL-2, IL-4, IL-8, IL-10, IL-12, IL-16, IL-18, and TNF-) caused by LPS stimulation in the cultured porcine alveolar macrophages. These results indicate that the recombinant porcine sTREM-1 protein can prevent TREM-1-mediated hyperinflammatory responses after exposure to LPS.
分类号: Q95
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