Expression and Purification of a Functional Porcine Soluble Triggering Receptor Expressed on Myeloid Cells 1
文献类型: 外文期刊
第一作者: Li, Hui
作者: Li, Hui;Yan, Leyan;Meng, Chunhua;Shi, Zhendan;Guo, Shuangshuang;Hu, Yiyi;He, Kongwang
作者机构:
关键词: Cytokines;Inflammatory responses;LPS;Porcine;TREM-1
期刊名称:ANIMAL BIOTECHNOLOGY ( 影响因子:2.282; 五年影响因子:1.702 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Triggering receptor expressed on myeloid cells 1 (TREM-1) plays a vital role in the pathogen-triggered amplification loop required for proinflammatory responses. Blockade of TREM-1 signaling may inhibit expansion of sepsis and prolong survival of animals. In the present study, the gene of porcine soluble TREM-1 was cloned and expressed in E. coli. After purification, the bioactivity of recombinant porcine soluble TREM-1 was tested in vitro on porcine alveolar macrophages. The results showed that supplementation with the recombinant porcine sTREM-1 protein rapidly and dose-dependently attenuated the upregulation of cytokines (IL-1, IL-2, IL-4, IL-8, IL-10, IL-12, IL-16, IL-18, and TNF-) caused by LPS stimulation in the cultured porcine alveolar macrophages. These results indicate that the recombinant porcine sTREM-1 protein can prevent TREM-1-mediated hyperinflammatory responses after exposure to LPS.
分类号: Q95
- 相关文献
作者其他论文 更多>>
-
A ratiometric fluorescent probe for the determination of quinolone antibiotics in milk based on N and S co-doped carbon quantum dots
作者:Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Wang, Fang;Li, Xiaoyun;Zhang, Yuchen;Li, Hui;Jiang, Shanxue;Li, Dandan;Yao, Zhiliang;Han, Jiajun;Gong, Wenwen
关键词:Carbon quantum dot; Ratiometric fluorescence probe; Moxifloxacin; Gatifloxacin; Ofloxacin; Milk
-
Near-gapless and haplotype-resolved apple genomes provide insights into the genetic basis of rootstock-induced dwarfing
作者:Li, Wei;Li, Hui;Sun, Haochen;Wang, Shiyao;Wang, Zijun;Li, Yuqi;Zhou, Bowen;Fan, Xingqiang;Xiong, Yao;Wang, Yi;Xu, Xuefeng;Han, Zhenhai;Chu, Chong;Zhang, Hengtao;Foster, Toshi M.;Lopez-Girona, Elena;Yu, Jiaxin;Li, Yi;Ma, Yue;Zhang, Ke;Zhang, Ke;Han, Yongming;Deng, Cecilia H.
关键词:
-
Risk assessment of broflanilide for human and non-target terrestrial organisms in cauliflower production
作者:Lin, Hongfang;Li, Na;Liu, Siyu;Yang, Lijing;Cheng, Yu;Sheng, Huishan;Li, Hui;Wen, Wanting;Guo, Yongze;Zhang, Yuting;Yang, Yuanping;Zhang, Yuting
关键词:Broflanilide; Cauliflower; Non-target terrestrial organisms; Safety evaluation
-
Effect of Tannic Acid on Antioxidant Function, Immunity, and Intestinal Barrier of Broilers Co-Infected with Coccidia and Clostridium perfringens
作者:Zhang, Zhengfan;Xu, Pengtao;Liu, Chengao;Chen, Jing;Ren, Bingbing;Guo, Shuangshuang;Li, Peng;Li, Lanlan;Ding, Binying;Du, Encun
关键词:antioxidant; broiler; intestinal barrier; necrotic enteritis; tannic acid
-
Acyl carrier protein OsMTACP2 confers rice cold tolerance at the booting stage
作者:Hou, Xiu-Li;Han, Xiangyan;Zhang, Wenqi;Yang, Chao;Li, Hui;Tang, Shanjie;Guo, Zhenhua;Liu, Chunyan;Zhang, Shaohua;Shui, Guanghou;Cao, Xiaofeng;Song, Xianwei;Han, Xiangyan;Meng, Ying;Wang, Lizhi;Zhang, Wenqi;Yang, Chao;Qin, Yongmei;Cao, Xiaofeng;Song, Xianwei
关键词:
-
Identification of the Effects of 5-Azacytidine on Porcine Circovirus Type 2 Replication in Porcine Kidney Cells
作者:Shan, Yiyi;Wu, Shenglong;Bao, Wenbin;Wu, Zhengchang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Wu, Zhengchang
关键词:5-Azacytidine; porcine circovirus type 2; porcine kidney cells
-
Research Note: Effects of different light-emitting diode lights during egg incubation on hatching performance and embryo development in White King pigeons
作者:Guo, Binbin;Zhu, Huanxi;Chen, Zhe;Yan, Leyan;Meng, Jun;Zhaorigetu
关键词:incubation; light; hatching time; embryo development; pigeon