Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus

文献类型： 外文期刊

第一作者： Li, Huawei

作者： Li, Huawei;Ji, Pengchao;Zhou, Enmin;Zhang, Gaiping;Yang, Jifei;Bao, Dengke;Hou, Jie;Zhi, Yubao;Yang, Yanyan;Qiao, Songlin;Zhang, Gaiping;Zhang, Gaiping;Li, Huawei

作者机构：

关键词： enzyme-linked immunosorbent assay;immunochromatography;porcine reproductive and respiratory syndrome

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnostic purposes in the field.

分类号： S8

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