Cold exposure induces the acquisition of brown adipocyte gene expression profiles in cattle inguinal fat normalized with a new set of reference genes for qRT-PCR

文献类型： 外文期刊

第一作者： Cao, K. X.

作者： Cao, K. X.;Hao, D.;Wang, J.;Peng, W. W.;Yan, Y. J.;Chen, H.;Cao, H. X.;Sun, F.

作者机构：

关键词： Cattle;Reference gene;Browning;White adipose tissue;Brite

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： AbstractThe last few years have seen great advances in our understanding of browning in white adipose tissue (WAT) where white adipocytes take on characteristics of brown adipocytes. At present, the economic significance of browning for animal husbandry is beginning to be realized with the emerging evidence that browning affects body weight not only in human and rodent but in farm animals. Quantitative RT-PCR provides a quick and sensitive way to preliminary determine browning of WAT. However, there have been no established condition specific reference genes for browning of cattle WAT. As the results showed, the most two stable reference genes for diet treatment wereWdr33(M=0.38) andHdac3(M=0.43), while the most three internal controls for temperature treatment wereHdac3(M=0.28),Wdr33(M=0.32), andHprt1(M=0.39) among the ten candidates. The mRNA relative expression levels of selective marker genes were normalized by normalization factor (geometric mean of control genes quantities). Cold exposure rather than high energy diet induced transcript elevations of brite specific markers (Cited1,Tbx1), thermoregulatory markers (brown and beigeversuswhite markers,i.e.,Cidea,Cox7a1,Ucp1), mitochondrial biogenesis markers (Nrf1,Nrf2,Tfam), and transcription regulators (brown and beigeversuswhite markers,i.e.,Pgc1α) (P<0.05) in cattle inguinal fat (iWAT). Quantitative RT-PCR is a preliminary study for WAT browning. In conclusion, cattle inguinal fat acquired brown adipocyte gene expression features upon cold acclimation with prerequisite identification of stable reference genes.Highlights?Two novel sets of reference genes were identified for diets and temperature treatments.?Cattle inguinal fat acquired brite adipocyte gene expression features upon cold acclimation.?Cattle backfat did not get brite adipocyte gene expression features upon cold or diet acclimation.]]>

分类号： S85

  • 相关文献

[1]Cidea controls lipid droplet fusion and lipid storage in brown and white adipose tissue. Wu LiZhen,Zhou LinKang,Gong JingYi,Li Peng,Wu LiZhen,Zhou LinKang,Gong JingYi,Li Peng,Chen Cheng,Xu Li,Ye Jing,Li De. 2014

[2]Two Endogenous Substrates for Polyphenoloxidase in Pericarp Tissues of Postharvest Rambutan Fruit. Su, Weiqiang,Peng, Hongxiang,Zhu, Jianhua,Xu, Liangxiong,Marti Bruna, Nuria. 2010

[3]Variations in contents of (-)-epicatechin and activities of phenylalanine ammonialyase and polyphenol oxidase of longan fruit during development. Yu, Chunyan,Sun, Jian,Yang, Bao,Jiang, Yueming,Xiang, Xu. 2010

[4]Effect of high hydrostatic pressure on overall quality parameters of watermelon juice. Liu, Y.,Zhao, X. Y.,Hu, X. S.. 2013

[5]Quality of fresh-cut Iceberg lettuce and spinach irradiated at doses up to 4 kGy. Fan, Xuetong,Sokorai, Kimberly J. B.,Guan, Wenqiang. 2012

[6]Effect of High Oxygen Modified Atmosphere Packaging on Browning Characteristics of Fresh-cut 'Fuji' Apple. Zhou, Yanwen,Wan, Sen,Li, Xihong,Xue, Ting. 2011

[7]Molecular Cloning and Expression of Polyphenoloxidase Genes from the Mushroom, Agaricus bisporus. Li Nan-yi,Qin Qiao-ping,Cai Wei-ming,Jin Qun-li,Ran Fu-lai. 2011

[8]Comparison of thermal, ultraviolet-c, and high pressure treatments on quality parameters of watermelon juice. Zhang, Chao,Li, Wu,Xu, Yong,Ma, Yue,Zhao, Xiaoyan,Trierweiler, Bernhard,Butz, Peter,Ruefer, Corinna E..

[9]Effects of UV-C treatment on browning and the expression of polyphenol oxidase (PPO) genes in different tissues of &ITAgaricus bisporus&IT during cold storage. Lei, Jing,Li, Bingjuan,Guan, Wenqiang,Brennan, Charles S.,Peng, Bo,Zhang, Na,Yan, Ruixiang,Brennan, Charles S.,Gao, Haiyan. 2018

[10]Oxidation of (-)-epicatechin is a precursor of litchi pericarp enzymatic browning. Liu, Liang,Xu, Yujuan,Zhang, Mingwei,Xiao, Gengsheng,Cao, Shaoqian,Deng, Qianchun.

[11]Changes in browning-related components of apple slices during different stages of instant controlled pressure drop-assisted hot air drying (AD-DIC). Gao, Kun,Bi, Jinfeng,Wang, Xueyuan,Gao, Kun,Chen, Qinqin,Bi, Jinfeng,Liu, Xuan,Wu, Xinye,Wang, Xueyuan.

[12]Effects of low temperature conditioning on fruit quality and peel browning spot in 'Huangguan' pears during cold storage. Li, Dan,Shang, Zhonglin,Li, Dan,Cheng, Yudou,Guan, Junfeng,Doug, Yu.

[13]Characterization and Comparative Expression Profiling of Browning Response in Medinilla formosana after Cutting. Wang, Yan,Wang, Yiting,Song, Xijiao,Chen, Jianping,Liz, Kunfeng. 2016

[14]An effective route for the micropropagation of Medinilla formosana through ovary culture in vitro. Wang, Y.,Feng, D. D.,Chen, J. P.,Li, X. B.. 2015

[15]The effect of MAP on quality and browning of cold-stored plum fruits. Guan, JunFeng,Dou, ShiJuan. 2010

[16]Enzymatic Browning of Postharvest Litchi: a Review. Wang, J. B.,Wang, X. S.,Jin, Z. Q.. 2010

[17]Combination of Sodium Chlorite and Calcium Propionate Reduces Enzymatic Browning and Microbial Population of Fresh-Cut "Granny Smith" Apples. Fan, Xuetong,Guan, Wenqiang. 2010

[18]A method to suppress the browning in banana (Musa, AAA) embryogenic callus induced. Chang, S.,Shu, H.,Chang, S.,Shu, H.,Chang, S.. 2013

[19]Effects of high CO2 in-package treatment on flavor, quality and antioxidant activity of button mushroom (Agaricus bisporus) during postharvest storage. Lin, Qiong,Lu, Yuanyuan,Zhang, Jie,Liu, Wei,Wang, Zhidong,Guan, Wenqiang.

[20]Low intensity ultrasound as a pretreatment to drying of daylilies: Impact on enzyme inactivation, color changes and nutrition quality parameters. Zhang, Zhongyuan,Niu, Liying,Li, Dajing,Liu, Chunquan,Ma, Rui,Song, Jiangfeng,Zhao, Jiangtao,Ma, Rui.

作者其他论文 更多>>
  • Metabolite analysis of peach (Prunus persica L. Batsch) branches in response to freezing stress

    作者：Li, Y.;Wang, Y.;Wang, Z.;Liu, G.;Chang, R.;Chen, H.;Li, J.;Tian, Q.

    关键词：Freezing stress; Metabolic networks; Metabolism; Peach

  • Arginine requirement of breeding pigeons during incubation and lactation in the 2+4 lactation pattern

    作者：Zhang, W.;Zheng, S.;Huang, W.;Lu, X.;Lu, H.;Zhang, Y.;Lv, Y.;Li, Z.;Chen, W.;Mo, S.;Tan, G.;He, Y.;Huang, Q.;Li, Z.;Wang, W.;Huang, Y.;Peng, J.;Gou, Z.;Wang, J.;Wang, Z.;Li, Z.;Li, Z.;Xu, X.

    关键词：Arginine; reproductive performance; growth; oxidative stress; breeding pigeon

  • Dietary supplementation with tryptophan improves the production and growth performance of breeding pigeons under 2+4 model

    作者：Zheng, S.;Yan, K.;Tang, Y.;Zhang, W.;Zhang, Y.;Huang, W.;Yang, M.;Lu, H.;Li, Z.;He, Y.;Li, Z.;Gao, H.;Zhou, S.;Lv, Y.;Wang, W.;Huang, Y.;Peng, J.;Gou, Z.;Wang, Z.;Wang, J.;Li, Z.;Li, Z.;Xu, X.

    关键词：Tryptophan; breeding pigeons; squab; performance; reproductive metabolism

  • First Report of Fusarium Wilt on Lily (Lilium 'Tresor') Caused by Fusarium armeniacum in Jiangsu Province, China

    作者：Gao, J. L.;Liang, J. H.;Yang, F. P.;Wang, J.;Zhang, X. H.;Du, Y. P.;Sun, P. N.;Sun, J. G.

    关键词：China; Fusarium armeniacum; Fusarium wilt; Lilium 'Tresor'

  • First Report of Lasiodiplodia theobromae Causing Leaf Spot of Agave sisalana in Guangxi, China

    作者：Lin, A.;Lin, A.;Xu, P. D.;Chen, D.;Yi, J. F.;Chen, H.;Zhao, W. J.;Lin, A.;Xu, G.;Yi, K. X.;Xu, P. D.;Zhao, W. J.;Yi, J. F.;Qin, Q.

    关键词：Agave sisalana; Lasiodiplodia theobromae; leaf spot

  • EFFECTS OF MANNAN-OLIGOSACCHARIDES ON GROWTH PERFORMANCE, SERUM BIOCHEMICAL INDICES, IMMUNE FUNCTION, AND INTESTINAL MORPHOLOGY IN FATTENING TAN SHEEP

    作者：Ma, L.;Zhao, Z.;Wang, J.;Ma, Q.;Ma, L.;Jiang, Q.;Tian, J.

    关键词：Tan sheep; growth performance; antioxidant; immune function; intestinal morphology

  • Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation

    作者：Tzanetakis, I. E.;Atanda, H. Y.;Osundahunsi, B.;Sierra-Mejia, A.;Singh, S.;Stainton, D.;Villamor, D. E.;Aknadibossian, V.;Folimonova, S. Y.;Spak, J.;Franova, J.;Koloniuk, I.;Constable, F.;Harper, S. J.;Hammond, J.;Jordan, R.;Candresse, T.;Marais, A.;Freitas-Astua, J.;Barbosa, C.;Girardi, E.;Laranjeira, F.;Stuchi, E.;Fuchs, M.;Jelkmann, W.;Zikeli, K.;Maliogka, V. I.;Katis, N.;Martin, R. R.;Mollov, D.;Wang, J.;Vidalakis, G.;Lavagi-Craddock, I.;Mitra, A.;Aboughanem-Sabanadzovic, N.;Al Rwahnih, M.;Alabi, O. J.;Alioto, D.;Minutolo, M.;Bagi, F.;Baranwal, V. K.;Sharma, S. K.;Bar-Joseph, M.;Varma, A.;Batista Le Riverend, L.;Belien, T.;Benitez-Galeano, M. J.;Bennypaul, H.;Gaafar, Y. Z. A.;Gratz, A.;Bertaccini, A.;Bester, R.;Maree, H. J.;Blouin, A. G.;Blystad, D. -R.;Hamborg, Z.;Botermans, M.;Roenhorst, A.;Schoen, R.;Westenberg, M.;Bozan, O.;Onelge, N.;Brakta, A.;Brans, Y.;Bulajic, A.;Caglayan, K.;Catara, A.;Choueiri, E.;Cieslinska, M.;Cook, G.;Cui, W.;Fiore, N.;da Graca, J.;Davino, S.;Panno, S.;Delmiglio, C.;Perez-Egusquiza, Z.;Tang, J.;Thompson, J.;Veerakone, S.;Dewdney, M. M.;Levy, A.;Shilts, T.;Dewdney, M. M.;Levy, A.;Shilts, T.;Di Serio, F.;Minafra, A.;Navarro, B.;Saldarelli, P.;Diaz-Lara, A.;Digiaro, M.;Djelouah, K.;Dong, Y. F.;Donovan, N.;Wulff, N. A.;Donovan, N.;Druciarek, T. Z.;Duran-Vila, N.;Moreno, P.;Navarro, L.;Olmos, A.;Ruiz-Garcia, A. B.;Vives, M. C.;Elci, E.;Ulubas Serce, C.;Esquivel-Farina, A.;Fall, M. L.;Fall, M. L.;Fan, X. D.;Figueroa, J.;Palacios, M. F.;Fowkes, A. R.;Fox, A.;Skelton, A.;Vazquez-Iglesias, I.;Fuchs, R.;Garcia, M. L.;Ghosh, D.;Glasa, M.;Gomez Talquenca, S.;Gritsenko, D.;Hajeri, S.;Hajizadeh, M.;Ho, T.;Holeva, M.;Varveri, C.;Holkar, S. K.;Horner, M.;Hurtado-Gonzales, O. P.;Ippolito, A.;Isac, V.;Iwanami, T.;Jofre-y-Garfias, A. E.;Konings, H.;Meekes, E. T. M.;Krizanac, I.;Krueger, R.;Kyrychenko, A.;Licciardello, G.;Lu, Q. -Y.;MacFarlane, S. A.;Marcone, C.;Margaria, P.;Menzel, W.;Winter, S.;Martic, A.;Massart, S.;Tahzima, R.;Mathioudakis, M. M.;Matic, S.;Turina, M.;Mavric Plesko, I.;Mehle, N.;Melzer, M. J.;Meng, B.;Miljanic, V.;Stajner, N.;Nerva, L.;Okic, A.;Pallas, V.;Poudel-Ward, B.;Wright, G.;Radisek, S.;Ramos-Gonzalez, P. L.;Ramteke, P.;Ranabhat, N. B.;Rivarez, M. P. S.;Rivas, F.;Roy, A.;Sabanadzovic, S.;Safarova, D.;Salem, N.;Sanahuja Solsona, G.;Umble, J.;Skoric, D.;Starovic, M.;Svoboda, P.;Tessitori, M.;Thermoz, J. -P.;Thompson, E.;Trebicki, P.;Valiunas, D.;Varallyay, E.;Vasquez-Gutierrez, U.;Voncina, D.;Wetzel, T.;Xu, W. X.;Yokomi, R.;Zhou, C.;Zindovic, J.

    关键词：fruit; ornamentals; small fruits; tree fruits; viruses and viroids

微信小程序