Development of a high-performance liquid chromatography method for the determination of florfenicol in animal feedstuffs

文献类型： 外文期刊

第一作者： Yang, JinJing

作者： Yang, JinJing;Huang, LingLi;Sun, GuiZhi;Ke, XianBing;Yang, Bo;Qian, MingRong

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关键词： High-performance liquid chromatography;Thin layer chromatography;Florfenicol;Animal feedstuffs

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

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收录情况： SCI

摘要： Highlights?A TLC-HPLC method was developed for determining florfenicol in animal feedstuffs.?The analytical method was proved to be reliable, simple and cost-effective.?TLC could be used as an alternative purification technique for complex matrix.AbstractAn effective thin layer chromatography (TLC) purification procedure coupled to high-performance liquid chromatography (HPLC) method was developed for the determination of florfenicol (FF) in pig, chicken and fish feedstuffs. The feedstuff samples were extracted with ethyl acetate, defatted with n-hexane saturated with acetonitrile, and further purified by TLC. The chromatographic separation was performed on a Waters Symmetry C18column using an isocratic procedure with acetonitrile-water (35:65, v/v) at 0.6mL/min. The ultraviolet (UV) detector was set at a wavelength of 225nm. The FF concentrations in feedstuff samples were quantified using a standard curve. Good linear correlations (y=159075x?15054,r>0.9999) were achieved within the concentration range of 0.05–200μg/mL. The recoveries of FF spiked at levels of 1, 100 and 1000μg/g ranged from 80.6% to 105.3% with the intra-day and inter-day relative standard deviation (RSD) less than 9.3%. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.02 and 0.06mg/kg for pig feedstuffs, 0.02 and 0.07mg/kg for chicken feedstuffs, and 0.02 and 0.05mg/kg for fish feedstuffs, respectively. This reliable, simple and cost-effective method could be applied to the routine monitoring of FF in animal feedstuffs.]]>

分类号： Q503

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