Engineering of Yersinia Phytases to Improve Pepsin and Trypsin Resistance and Thermostability and Application Potential in the Food and Feed Industry

文献类型： 外文期刊

第一作者： Niu, Canfang

作者： Niu, Canfang;Yang, Peilong;Luo, Huiying;Huang, Huoqing;Wang, Yaru;Yao, Bin

作者机构：

关键词： phytases;pepsin resistance;trypsin resistance;thermostability;stability at low pH;hydrolysis of corn meal phytate;optimizing the polarity and charge of the amino acids

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Susceptibility to proteases usually limits the application of phytase. We sought to improve the pepsin and trypsin resistance of YeAPPA from Yersinia enterocolitica and YkAPPA from Y. kristensenii by optimizing amino acid polarity and charge. The predicted pepsin/trypsin cleavage sites F89/K226 in pepsin/trypsin-sensitive YeAPPA and the corresponding sites (F89/E226) in pepsin-sensitive but trypsin-resistant YkAPPA were substituted with S and H, respectively. Six variants were produced in Pichia pastoris for catalytic and biochemical characterization. F89S, E226H, and F89S/E226H elevated pepsin resistance and thermostability and K226H and F89S/K226H improved pepsin and trypsin resistance and stability at 60 degrees C and low pH. All the variants increased the ability of the proteins to hydrolyze phytate in corn meal by 2.6-14.9-fold in the presence of pepsin at 37 degrees C and low pH. This study developed a genetic manipulation strategy specific for pepsin/trypsin-sensitive phytases that can improve enzyme tolerance against proteases and heat and benefit the food and feed industry in a cost-effective way.

分类号： R15`S

  • 相关文献

[1]A two-step discriminated method to identify thermophilic proteins. Tang, Hua,Cao, Ren-Zhi,Wang, Wen,Liu, Tie-Shan,Wang, Li-Ming,He, Chun-Mei. 2017

[2]An acid and highly thermostable xylanase from Phialophora sp G5. Zhang, Fan,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Yuan, Tiezheng,Huang, Huoqing,Yang, Peilong,Yao, Bin,Zhang, Fan,Miao, Lihong. 2011

[3]Purification and characterization of a psychrophilic catalase from Antarctic Bacillus. Wang, Wei,Sun, Mi,Zhang, Bin,Liu, Wanshun. 2008

[4]An intramolecular disulfide bond is required for the thermostability of methyl parathion hydrolase, OPHC2. Chu, Xiao-yu,Tian, Jian,Wu, Ning-feng,Fan, Yun-liu.

[5]Heterologous expression of a gene encoding a thermostable beta-galactosidase from Alicyclobacillus acidocaldarius. Yuan, Tiezheng,Yang, Peilong,Wang, Yaru,Meng, Kun,Luo, Huiying,Yao, Bin,Zhang, Wei,Wu, Ningfeng,Fan, Yunliu.

[6]The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Wang, Guozeng,Wu, Jingjing,Lin, Juan,Ye, Xiuyun,Yao, Bin.

[7]A novel, alkali-tolerant thermostable xylanase from Saccharomonospora viridis: direct gene cloning, expression and enzyme characterization. Wang, Ziyuan,Jin, Yi,Wu, Huijun,Xie, Xiangming,Tian, Zhaofeng,Wu, Yuying.

[8]Improvement of alkali stability and thermostability of Paenibacillus campinasensis Family-11 xylanase by directed evolution and site-directed mutagenesis. Zheng, Hongchen,Liu, Yihan,Han, Yang,Lu, Fuping,Zheng, Hongchen,Liu, Yihan,Sun, Mingzhe,Han, Yang,Wang, Jianling,Lu, Fuping,Zheng, Hongchen,Sun, Junshe,Liu, Yihan,Wang, Jianling,Sun, Mingzhe,Lu, Fuping.

[9]Structure-Based Design of Mucor pusillus Pepsin for the Improved Ratio of Clotting Activity/Proteolytic Activity in Cheese Manufacture. Zhang, Jie,Sun, Yonghai,Luo, Quan,Zhang, Jie,Li, Zhuolin,Li, Tiezhu,Wang, Tuoyi.

[10]Genetic and biochemical characterization of a protease-resistant mesophilic beta-mannanase from Streptomyces sp S27. Shi, Pengjun,Yuan, Tiezheng,Zhao, Junqi,Huang, Huoqing,Luo, Huiying,Meng, Kun,Wang, Yaru,Yao, Bin.

[11]IMPROVEMENT ON THE THERMOSTABILITY AND ACTIVITY OF APX1 FROM ENERGY PLANT JATROPHA CURCAS L. BY HYPER-ACIDIC FUSION PARTNERS. Zhang, Mengru,Yang, Yumei,Li, Xujuan,Wang, Haibo,Yang, Shuanglong,Wang, Shasha,Gong, Ming,Zou, Zhurong,Li, Xujuan,Wang, Haibo. 2014

[12]A novel thermophilic endo-beta-1,4-mannanase from Aspergillus nidulans XZ3: functional roles of carbohydrate-binding module and Thr/Ser-rich linker region. Lu, Haiqiang,Luo, Huiying,Shi, Pengjun,Huang, Huoqing,Meng, Kun,Yang, Peilong,Yao, Bin. 2014

[13]Structural Insights into the Thermophilic Adaption Mechanism of Endo-1,4-beta-Xylanase from Caldicellulosiruptor owensensis. Liu, Xin,Xin, Fengjiao,Wen, Boting,Gu, Tianyi,Wang, Fengzhong,Sun, Lichao,Liu, Tengfei,Shi, Xinyuan,Zhang, Yuebin,Mi, Shuofu. 2018

[14]Concurrent mutations in six amino acids in beta-glucuronidase improve its thermostability. Xiong, Ai-Sheng,Peng, Ri-He,Cheng, Zong-Ming,Li, Yi,Liu, Jin-Ge,Zhuang, Jing,Gao, Feng,Xu, Fang,Qiao, Yu-Shan,Zhang, Zhen,Chen, Jian-Min,Yao, Quan-Hong. 2007

[15]Two neutral thermostable cellulases from Phialophora sp G5 act synergistically in the hydrolysis of filter paper. Zhao, Junqi,Shi, Pengjun,Li, Zhongyuan,Yang, Peilong,Luo, Huiying,Bai, Yingguo,Wang, Yaru,Yao, Bin. 2012

[16]Improving the thermostability of methyl parathion hydrolase from Ochrobactrum sp M231 using a computationally aided method. Tian, Jian,Wang, Ping,Huang, Lu,Chu, Xiaoyu,Wu, Ningfeng,Fan, Yunliu. 2013

[17]Improved thermal performance of Thermomyces lanuginosus GH11 xylanase by engineering of an N-terminal disulfide bridge. Wang, Yawei,Fu, Zheng,Xiong, Hairong,Wang, Yawei,Fu, Zheng,Huang, Huoqing,Yao, Bin,Zhang, Huashan,Turunen, Ossi.

[18]Gene cloning, expression, and characterization of a thermostable xylanase from Nesterenkonia xinjiangensis CCTCC AA001025. Kui, Hong,Luo, Huiying,Shi, Pengjun,Bai, Yingguo,Yuan, Tiezheng,Wang, Yaru,Yang, Peilong,Yao, Bin,Kui, Hong,Dong, Shouliang.

[19]High efficiency and throughput system in directed evolution in vitro of reporter gene. Xiong, Ai-Sheng,Peng, Ri-He,Liu, Jin-Ge,Zhuang, Jing,Qiao, Yu-Shan,Xu, Fang,Cai, Bing,Zhang, Zhen,Chen, Jian-Min,Yao, Quan-Hong.

[20]A novel thermoacidophilic and thermostable endo-beta-1,4-glucanase from Phialophora sp G5: its thermostability influenced by a distinct beta-sheet and the carbohydrate-binding module. Zhao, Junqi,Shi, Pengjun,Huang, Huoqing,Li, Zhongyuan,Yuan, Tiezheng,Yang, Peilong,Luo, Huiying,Bai, Yingguo,Yao, Bin. 2012

作者其他论文 更多>>

微信小程序