Engineering of Yersinia Phytases to Improve Pepsin and Trypsin Resistance and Thermostability and Application Potential in the Food and Feed Industry
文献类型: 外文期刊
第一作者: Niu, Canfang
作者: Niu, Canfang;Yang, Peilong;Luo, Huiying;Huang, Huoqing;Wang, Yaru;Yao, Bin
作者机构:
关键词: phytases;pepsin resistance;trypsin resistance;thermostability;stability at low pH;hydrolysis of corn meal phytate;optimizing the polarity and charge of the amino acids
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Susceptibility to proteases usually limits the application of phytase. We sought to improve the pepsin and trypsin resistance of YeAPPA from Yersinia enterocolitica and YkAPPA from Y. kristensenii by optimizing amino acid polarity and charge. The predicted pepsin/trypsin cleavage sites F89/K226 in pepsin/trypsin-sensitive YeAPPA and the corresponding sites (F89/E226) in pepsin-sensitive but trypsin-resistant YkAPPA were substituted with S and H, respectively. Six variants were produced in Pichia pastoris for catalytic and biochemical characterization. F89S, E226H, and F89S/E226H elevated pepsin resistance and thermostability and K226H and F89S/K226H improved pepsin and trypsin resistance and stability at 60 degrees C and low pH. All the variants increased the ability of the proteins to hydrolyze phytate in corn meal by 2.6-14.9-fold in the presence of pepsin at 37 degrees C and low pH. This study developed a genetic manipulation strategy specific for pepsin/trypsin-sensitive phytases that can improve enzyme tolerance against proteases and heat and benefit the food and feed industry in a cost-effective way.
分类号: R15`S
- 相关文献
作者其他论文 更多>>
-
Fluorescent Probes Based on Ag NPs@N/GQDs and Molecularly Imprinted Polymer for Sensitive Detection of Noradrenaline in Bananas
作者:Wang, Yaru;Wang, Mingyue;Liu, Sixin;Wang, Yaru;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Wu, Yuwei;Wang, Mingyue;Wang, Yaru;Li, Shuhuai;Wang, Mingyue;Li, Bei
关键词:Fluorescent probe; Graphene quantum dots; Silver nanoparticles; Molecular imprinted polymer; Noradrenaline
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者:Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词:high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者:Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词:
-
Cooperative Fermentation Using Multiple Microorganisms and Enzymes Potentially Enhances the Nutritional Value of Spent Mushroom Substrate
作者:Zhang, Anrong;He, Weizhao;Han, Yunsheng;Zheng, Aijuan;Chen, Zhimin;Meng, Kun;Yang, Peilong;Liu, Guohua
关键词:fermentation; Pleurotus eryngii; nutritional value; condition optimization; enzymatic treatment
-
Comparative assessment of morphological, cytological, and reproductive characteristics of the spontaneous mixoploid 'MM 2 x /4 x' and its diploid counterpart 'Miss Manilla ' in Bougainvillea ( Bougainvillea x buttiana Honlttum & Standl.)
作者:Li, Haiyan;Leng, Qingyun;Sun, Luping;Lu, Jinping;Xu, Shisong;Wang, Yaru;Liu, Shaoshan;Yin, Junmei;Niu, Junhai;Li, Haiyan;Wang, Yaru;Yin, Junmei;Niu, Junhai;Leng, Qingyun;Wang, Yaru;Niu, Junhai;Yin, Junmei;Lopez, Hernan Ariel;Lopez, Hernan Ariel;Ma, Chao
关键词:Bougainvillea; Mixoploid; Morphological traits; Cytological analysis; Hybridization; Polyploid breeding
-
Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization
作者:Zhang, Ruohan;Zhang, Honglian;Tu, Tao;Luo, Huiying;Yao, Bin;Tian, Jian;Huang, Huoqing;Guan, Feifei;Tian, Xiaoqian;Zhang, Yan;Wu, Ningfeng;Tian, Jian;Tian, Xiaoqian;Zhang, Yan;Sun, Jilu
关键词:Chitin; Chitinase; Endo-activity; Mutants; Functional oligosaccharides
-
Exploiting Systematic Engineering of the Expression Cassette as a Powerful Tool to Enhance Heterologous Gene Expression in Trichoderma reesei
作者:Ji, Wangli;Xu, Xinxin;Zhang, Wei;Xu, Li;Sun, Xianhua;Zhang, Honglian;Luo, Huiying;Yao, Bin;Su, Xiaoyun;Huang, Huoqing
关键词:heterologous gene expression; signal peptide; Kozak sequence; 3 '-UTR; Trichoderma reesei; mannanase