Development and application of a molecular marker for TMV resistance based on N gene in tobacco (Nicotiana tabacum)

文献类型： 外文期刊

第一作者： Zhang Yu

作者： Zhang Yu;Wen Liuying;Yang Aiguo;Luo Chenggang;Cheng Lirui;Jiang Caihong;Chang Aixia;Wang Yuanying;Li Wei;Zhang Jing;Xiao Zhixin

作者机构： Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Genet Improvement & Biotechnol, 11 Ke Yuan Rd, Qingdao 266101, Peoples R China;Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Genet Improvemen

关键词： N gene;TMV;Molecular marker;Breeding;Tobacco

期刊名称：EUPHYTICA （ 2020影响因子：1.895； 五年影响因子：2.181 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Tobacco mosaic virus (TMV) caused serious loss in yield and quality of tobacco every year. It is a long-term goal to improve the tobacco resistance against TMV by tobacco breeding. N gene was the firstly reported TMV-resistant gene, which showed resistance against all Tobamoviruses except the Ob stain and belonged to the toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat class of plant resistance (R) genes. At present, N gene had already been widely used in tobacco conventional breeding, but there is rare available molecular maker used in marker-assisted selection of TMV resistance. In this study, we designed a pair of primers that specific amplify N gene fragment based on the sequence of N gene intron III, named N-marker. Then, we identified TMV resistance by two selecting methods, PCR with N-marker and inoculated with the TMV-C strain. Results from the two method showed that (1) 13 varieties among 67 tobacco varieties displayed hypersensitive reaction when inoculated with the TMV-C strain, also contained N gene fragments screened by PCR with N-marker; (2) 105 strains of 200 BC1 strains showed resistance against TMV when inoculated with TMV-C strain, meanwhile, 103 of the 105 strains contained N gene fragment verified by PCR with N-marker. Therefore, the N-marker is reliable for high throughput screening of germplasm resources and tobacco breeding materials in selection of N-mediated TMV resistance. Our study not only developed a molecular marker for tobacco breeding, but also identified new germplasm resources that are resistant to TMV.

分类号： S3

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