Knockdown of β-conglycinin α′ and α subunits alters seed protein composition and improves salt tolerance in soybean

文献类型： 外文期刊

第一作者： Yang, Rufei

作者： Yang, Rufei;Yang, Zhongyi;Pu, Yixiang;Liu, Mengyu;Du, Jingyi;Xu, Zhiri;Xu, Zefei;Yu, Deyue;Kan, Guizhen;Ma, Yujie;Zhang, Shanshan;Zhang, Hengyou;Zhang, Wei

作者机构：

关键词： soybean (Glycine max [L.] Merr.); beta-conglycinin (7S); alpha and alpha ' subunits; sulfur-containing amino acids; seed protein content; salt tolerance

期刊名称：PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：7.0 ）

ISSN： 0960-7412

年卷期： 2024 年 120 卷 4 期

页码：

收录情况： SCI

摘要： Soybean is an important plant source of protein worldwide. Increasing demands for soybean can be met by improving the quality of its seed protein. In this study, GmCG-1, which encodes the beta-conglycinin alpha ' subunit, was identified via combined genome-wide association study and transcriptome analysis. We subsequently knocked down GmCG-1 and its paralogues GmCG-2 and GmCG-3 with CRISPR-Cas9 technology and generated two stable multigene knockdown mutants. As a result, the beta-conglycinin content decreased, whereas the 11S/7S ratio, total protein content and sulfur-containing amino acid content significantly increased. Surprisingly, the globulin mutant exhibited salt tolerance in both the germination and seedling stages. Little is known about the relationship between seed protein composition and the salt stress response in soybean. Metabonomics and RNA-seq analysis indicated that compared with the WT, the mutant was formed through a pathway that was more similar to that of active salicylic acid biosynthesis; however, the synthesis of cytokinin exhibited greater defects, which could lead to increased expression of plant dehydrin-related salt tolerance proteins and cell membrane ion transporters. Population evolution analysis suggested that GmCG-1, GmCG-2, and GmCG-3 were selected during soybean domestication. The soybean accessions harboring GmCG-1(Hap1 )presented relatively high 11S/7S ratios and relatively high salt tolerance. In conclusion, knockdown of the beta-conglycinin alpha and alpha ' subunits can improve the nutritional quality of soybean seeds and increase the salt tolerance of soybean plants, providing a strategy for designing soybean varieties with high nutritional value and high salt tolerance.

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