Deciphering the differential expression patterns of yield-related negative regulators in hexaploid wheat cultivars and hybrids at different growth stages

文献类型： 外文期刊

第一作者： Awan, Muhammad Jawad Akbar

作者： Awan, Muhammad Jawad Akbar;Farooq, Muhammad Awais;Naqvi, Rubab Zahra;Waqas, Muhammad Abu Bakar;Mahmood, Muhammad Arslan;Buzdar, Muhammad Ismail;Amin, Imran;Saeed, Nasir A.;Mansoor, Shahid;Farooq, Muhammad Awais;Karamat, Umer;Bukhari, Sayyad Ali Raza;Rasheed, Awais;Rasheed, Awais;Rasheed, Awais;Mansoor, Shahid;Bukhari, Sayyad Ali Raza;Karamat, Umer

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关键词： Heterosis; Hybrid vigor; Cytoplasm swapping; Transcriptional expression profiling; Syntenic triads; Homeologs

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 0301-4851

年卷期： 2024 年 51 卷 1 期

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收录情况： SCI

摘要： BackgroundHexaploid bread wheat underwent a series of polyploidization events through interspecific hybridizations that conferred adaptive plasticity and resulted in duplication and neofunctionalization of major agronomic genes. The genetic architecture of polyploid wheat not only confers adaptive plasticity but also offers huge genetic diversity. However, the contribution of different gene copies (homeologs) encoded from different subgenomes (A, B, D) at different growth stages remained unexplored.MethodsIn this study, hybrid of elite cultivars of wheat were developed via reciprocal crosses (cytoplasm swapping) and phenotypically evaluated. We assessed differential expression profiles of yield-related negative regulators in these cultivars and their F1 hybrids and identified various cis-regulatory signatures by employing bioinformatics tools. Furthermore, the preferential expression patterns of the syntenic triads encoded from A, B, and D subgenomes were assessed to decipher their functional redundancy at six different growth stages.ResultsHybrid progenies showed better heterosis such as up to 17% increase in the average number of grains and up to 50% increase in average thousand grains weight as compared to mid-parents. Based on the expression profiling, our results indicated significant dynamic transcriptional expression patterns, portraying the different homeolog-dominance at the same stage in the different cultivars and their hybrids. Albeit belonging to same syntenic triads, a dynamic trend was observed in the regulatory signatures of these genes that might be influencing their expression profiles.ConclusionThese findings can substantially contribute and provide insights for the selective introduction of better cultivars into traditional and hybrid breeding programs which can be harnessed for the improvement of future wheat.

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