AwSclB regulates a network for Aspergillus westerdijkiae asexual sporulation and secondary metabolism independent of the fungal light control

文献类型： 外文期刊

第一作者： Wang, Gang

作者： Wang, Gang;Liu, Yibing;Hu, Yafan;Wei, Zifan;Tai, Bowen;Yang, Bolei;Xing, Fuguo;Pan, Jiaqi;Li, Erfeng;Xing, Fuguo

作者机构：

关键词： Aspergillus westerdijkiae; Ochratoxin A; Light control; Asexual sporulation; Secondary metabolism

期刊名称：FUNGAL GENETICS AND BIOLOGY （ 影响因子：3.0； 五年影响因子：3.3 ）

ISSN： 1087-1845

年卷期： 2024 年 171 卷

页码：

收录情况： SCI

摘要： As a prevalent pathogenic fungus, Aspergillus westerdijkiae poses a threat to both food safety and human health. The fungal growth, conidia production and ochratoxin A (OTA) in A. weterdijkiae are regulated by many factors especially transcription factors. In this study, a transcription factor AwSclB in A. westerdijkiae was identified and its function in asexual sporulation and OTA biosynthesis was investigated. In addition, the effect of light control on AwSclB regulation was also tested. The deletion of AwSclB gene could reduce conidia production by downregulation of conidia genes and increase OTA biosynthesis by up -regulation of cluster genes, regardless under light or dark conditions. It is worth to note that the inhibitory effect of light on OTA biosynthesis was reversed by the knockout of AwSclB gene. The yeast one -hybrid assay indicated that AwSclB could interact with the promoters of BrlA, ConJ and OtaR1 genes. This result suggests that AwSclB in A. westerdijkiae can directly regulate asexual conidia formation by activating the central developmental pathway BrlA-AbaA-WetA through upregulating the expression of AwBrlA, and promote the light response of the strain by activating ConJ. However, AwSclB itself is unable to respond to light regulation. This finding will deepen our understanding of the molecular regulation of A. westerdijkiae development and secondary metabolism, and provide potential targets for the development of new fungicides.

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