A method for the rapid determination of pesticides coupling thin-layer chromatography and enzyme inhibition principles

文献类型： 外文期刊

第一作者： Shao, Yunling

作者： Shao, Yunling;Wang, Miao;Cao, Jing;She, Yongxin;Cao, Zhen;Jin, Fen;Wang, Jing;Shao, Yunling;Wang, Miao;Cao, Jing;She, Yongxin;Cao, Zhen;Jin, Fen;Wang, Jing;Hao, Zhenxia;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.

作者机构：

关键词： Enzyme inhibition; Thin-layer chromatography (TLC); Pesticides; Paper-based chip

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 416 卷

页码：

收录情况： SCI

摘要： Herein, we developed a method coupling TLC and enzyme inhibition principles to rapidly detect OPs (dichlorvos, paraoxon and parathion). After the removal of the organic solvent from the samples using TLC and paper-based chips, the enzyme was added to the detection system. The results showed that the current method effectively reduced the effects of solvents on enzyme behavior. Moreover, the pigments could be successfully retained on TLC with 40% ddH2O/ACN solution (v/v) as a developing solvent. Additionally, the detection limits (LODs) were 0.002 mu g/mL for dichlorvos, 0.006 mu g/mL for paraoxon, and 0.003 mu g/mL for parathion. Finally, the method was applied to spiked cabbage, cucumber, and spinach and showed good average recoveries ranging between 70.22% and 119.79%. These results showed that this paper-based chip had high sensitivity, precleaning, and elimination of organic solvent properties. Furthermore, it provides a valuable idea for sample pretreatment and rapid determination of pesticide residues in food.

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