Investigating the mechanisms of flucarbazone-sodium resistance in Bromus japonicus Thunb

文献类型： 外文期刊

第一作者： Lan, Yuning

作者： Lan, Yuning;Xue, Xinhui;Cui, Hailan;Wang, Ruolin;Sun, Ying;Huang, Hongjuan;Wei, Shouhui;Li, Xiangju;Huang, Zhaofeng

作者机构：

关键词： Bromus japonicus; Flucarbazone-sodium; Herbicide metabolic resistance; Cytochrome P450; Glutathione S-transferase

期刊名称：PLANT AND SOIL （ 影响因子：4.1； 五年影响因子：4.7 ）

ISSN： 0032-079X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Background Bromus japonicus Thunb. is a hazardous weed in wheat fields in China. One B. japonicus population, HB-1, which was collected in Hebei Province, survived field rate flucarbazone-sodium exposure. Method To investigate the mechanism of flucarbazone-sodium resistance in the HB-1 population, the herbicide target als gene was isolated, and the in vitro ALS activity was tested. HPLC-MS/MS was applied to detect flucarbazone-sodium absorption and residue level. Furthermore, RNA-seq and qRT-PCR were applied to detect genes related to flucarbazone-sodium metabolism or flucarbazone-sodium and its metabolite transport. Result The results indicated that in resistant population there were no mutations in the als gene sequences or differences in the IC50 of ALS compared with the sensitive population. In addition, als gene relative copy number and expression also showed no difference. However, significantly increased herbicide metabolism was found in the HB-1 population. In the end, four cytochrome P450 genes (C71D55b, C71A1, C71D55, T5H), three glutathione S-transferase genes (GSTZ, GSTU6b, GSTFB), and three ABC transporter genes (ABCC3, ABCF1, ABCG11) were selected and identified as potentially playing a role in flucarbazone-sodium resistance.

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