Mediating a host cell signaling pathway linked to overwinter mortality offers a promising therapeutic approach for improving bee health

文献类型： 外文期刊

第一作者： Zhang, Yi

作者： Zhang, Yi;Han, Ri Chou;Zhang, Yi;Liu, Andrew;Huang, Shao Kang;Evans, Jay D.;Cook, Steve C.;Palmer-Young, Evan;Corona, Miguel;Alburaki, Mohamed;Li, Wen Feng;Hao, Yue;Banmeke, Olubukola;Posada-Florez, Francisco J.;Sadzewicz, Alex M.;Hamilton, Michele;Chen, Yan Ping;Zhang, Yi;Huang, Shao Kang;Liu, Ge;Gao, Ya Hui;Hao, Yue;Li, Ji Lian;Gilligan, Todd M.;Smith-Pardo, Allan H.;DeGrandi-Hoffman, Gloria;Xie, Hui Chun

作者机构：

关键词： Honey bee; Overwintering; Sirtuin; Metabolism; Biomarkers; Therapeutics

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：10.7； 五年影响因子：11.4 ）

ISSN： 2090-1232

年卷期： 2023 年 53 卷

页码：

收录情况： SCI

摘要： Introduction: Honey bees provides valuable pollination services for world food crops and wild flowering plants which are habitats of many animal species and remove carbon dioxide from the atmosphere, a powerful tool in the fight against climate change. Nevertheless, the honey bee population has been declining and the majority of colony losses occur during the winter. Objectives: The goal of this study was to understand the mechanisms underlying overwinter colony losses and develop novel therapeutic strategies for improving bee health. Methods: First, pathogen prevalence in overwintering bees were screened between 2015 and 2018. Second, RNA sequencing (RNA-Seq) for transcriptional profiling of overwintering honey bees was conducted and qRT-PCR was performed to confirm the results of the differential expression of selected genes. Lastly, laboratory bioassays were conducted to measure the effects of cold challenges on bee survivorship and stress responses and to assess the effect of a novel medication for alleviating cold stress in honey bees. Results: We identified that sirtuin signaling pathway is the most significantly enriched pathway among the down-regulated differentially expressed genes (DEGs) in overwintering diseased bees. Moreover, we showed that the expression of SIRT1 gene, a major sirtuin that regulates energy and immune metabolism, was significantly downregulated in bees merely exposed to cold challenges, linking cold stress with altered gene expression of SIRT1. Furthermore, we demonstrated that activation of SIRT1 gene expression by SRT1720, an activator of SIRT1 expression, could improve the physiology and extend the lifespan of cold-stressed bees. Conclusion: Our study suggests that increased energy consumption of overwintering bees for maintaining hive temperature reduces the allocation of energy toward immune functions, thus making the overwintering bees more susceptible to disease infections and leading to high winter colony losses. The novel information gained from this study provides a promising avenue for the development of therapeutic strategies for mitigating colony losses, both overwinter and annually. (c) 2023 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序