Development and application of a codominant marker of the melon rind colour gene CmAPRR2

文献类型： 外文期刊

第一作者： Ma, Jian

作者： Ma, Jian;Yuan, Guoliang;Zhang, Haijun;Qiu, Yanhong;Zong, Mei;Ma, Jian;Zhu, Xiuxiu;Zhang, Huijun

作者机构：

关键词： CmAPRR2; KASP; melon; molecular marker; rind colour

期刊名称：PLANT BREEDING （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 0179-9541

年卷期： 2024 年 143 卷 3 期

页码：

收录情况： SCI

摘要： Rind colour is an important quality attribute of melon appearance. Identifying target genes and developing functional molecular markers is significant for rind colour breeding in melon. This study involved a genetic analysis of the fruit rind colours of two inbred lines: H185 (with black green melon rind) and H160 (with white rind) alongside a fruit rind colour gene, CmAPRR2. The purpose was to discover the variation sites of the CmAPRR2 gene and develop specific molecular marker for rind colour in melon. The results showed that the black green rind is dominant over white, and single gene controls the colours. A mutation of the G856T base occurred in the eighth exon of the CmAPRR2 coding DNA region in H160, suggesting that this mutation is the key factor for the white rind colour. Thus, a codominant molecular marker, FC, for gene CmAPRR2 was developed and used for molecular identification of 189 F-2 individuals. The marker revealed a complete correspondence between genotype and phenotype. Additionally, the marker revealed that the other four white rinds had G856T mutations. This study provides a basis for targeted improvement of melon rind colours, offering a technical means for molecular marker-assisted breeding of melon rind colours.

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